Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Bratislava, Slovakia.
J Ind Microbiol Biotechnol. 2016 Sep;43(9):1333-44. doi: 10.1007/s10295-016-1800-4. Epub 2016 Jun 25.
In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.
在这项研究中，从针叶树红豆杉的根际中获得了一株微生物内生菌株，命名为链霉菌 AC35（FJ001754.1 链霉菌，GenBank）。16S rDNA 基因序列的高度相似性表明该菌株与 S. odorifer 密切相关。还进行了细胞内脂的主要脂肪酸图谱分析，以进一步鉴定该菌株。原子力显微镜和扫描声学显微镜用于对我们的菌株进行成像。提取其主要分泌物质，通过紫外可见光谱（UV-vis）、液相色谱-质谱（LC-MS/MS）和核磁共振进行评估，鉴定为生物活性异黄酮苷元-大豆苷元、大豆苷和染料木黄酮。在不同 pH 条件下进行的分批培养表明，当 pH 稳定在 7.0 时，抗霉素成分的产量会增加。通过 HPLC 检测到抗霉素，并通过 UV-vis 和 LC-MS/MS 结合多反应监测进行鉴定。我们的结果表明，链霉菌 AC35 可能被用作有效、具有药用活性化合物的潜在来源。
