Yoon Jung-Hoon, Park Sooyeon, Kang So-Jung, Jung Yong-Taek, Kim Wonyong
Korea Research Institute of Bioscience and Biotechnology, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2798-802. doi: 10.1099/ijs.0.010553-0. Epub 2009 Jul 23.
A Gram-positive-staining, non-motile and rod-shaped bacterium, strain ON10(T), was isolated from soil around a wastewater treatment plant in Korea and its taxonomic position was investigated by using a polyphasic approach. Strain ON10(T) grew optimally at pH 6.5-7.0 and 30 degrees C in the presence of 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ON10(T) clustered with the clade comprising Terrabacter species, with which it exhibited 16S rRNA gene sequence similarity values of 98.4-98.8 %. The cell-wall peptidoglycan type was based on ll-diaminopimelic acid and the cell-wall sugars were glucose, mannose, arabinose and xylose. The predominant menaquinone was MK-8(H(4)). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were iso-C(15 : 0) and iso-C(14 : 0). The DNA G+C content was 71.6 mol%. Strain ON10(T) exhibited DNA-DNA relatedness levels of 17-28 % to the type strains of Terrabacter species and could also be differentiated from these species by differences in phenotypic characteristics. On the basis of the data obtained, strain ON10(T) was considered to represent a novel species of the genus Terrabacter, for which the name Terrabacter terrigena sp. nov. is proposed. The type strain is ON10(T) (=KCTC 19602(T)=CCUG 57508(T)).
从韩国一家污水处理厂周围的土壤中分离出一株革兰氏阳性、无运动性的杆状细菌，菌株ON10(T)，并采用多相分类法研究其分类地位。菌株ON10(T)在pH 6.5 - 7.0和30℃、0.5%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株ON10(T)与包含地杆菌属物种的进化枝聚类，与之表现出98.4 - 98.8%的16S rRNA基因序列相似性值。细胞壁肽聚糖类型基于ll-二氨基庚二酸，细胞壁糖类为葡萄糖、甘露糖、阿拉伯糖和木糖。主要的甲基萘醌是MK-8(H4)。主要的极性脂是双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、一种未鉴定的磷脂和一种未鉴定的脂类。主要脂肪酸是异-C15:0和异-C14:0。DNA G+C含量为71.6 mol%。菌株ON10(T)与地杆菌属模式菌株的DNA-DNA相关性水平为17 - 28%，也可通过表型特征的差异与这些物种区分开来。根据获得的数据，菌株ON10(T)被认为代表地杆菌属的一个新物种，为此提出名为Terrabacter terrigena sp. nov.。模式菌株是ON10(T)（=KCTC 19602(T)=CCUG 57508(T)）。
