Baggio Carla Luiza, Silveira Aron Ferreira, Hyppolito Miguel Angelo
Hospital das Forças Armadas de Brasília.
Pro Fono. 2009 Apr-Jun;21(2):137-42. doi: 10.1590/s0104-56872009000200009.
gentamicin is an antibiotic that acts in Gram-negative bacilli infections, having as a side effect ototoxicity. Ototoxicity is an iatrogenic disturb provoked by drugs that modify the internal ear, affecting the cochlear and/or vestibular system and causing alterations in two important functions: equilibrium and audition. The main pediatric groups that receive aminoglicosides antibiotics are newborns who present serious infections in Neonate intensive care units.
to verify the occurrence of external ciliary cells (ECC) caused by gentamicin with single dose schemas of 4 mg/kg/day and 2,5mg/kg/day every 12 hours, through a morphological - scanning electronic microscopy (SEM) and functional - distortion product otoacoustic emissions (DPOAE) experimental study.
26 albino guinea pigs were evaluated through DPOAE pre and post gentamicin treatment. The guinea pigs were sacrificed in the programmed time after the intramuscular administration of the drugs for anatomic analysis using MEV.
the evaluation of the functional state of the ECC indicated preservation of the DPOAE in all of the guinea pigs. The SEM results, after being photographed were analyzed in terms of the number of the ECC in the cochlear basal turn in a specific photographic field.
lesions or alterations in the functioning of the ECC of albino guinea-pigs after the use of 4 mg/Kg/day and 2,5mg/Kg/day every 12 hours for a period 10 and 14 days were not observed.
庆大霉素是一种用于革兰氏阴性杆菌感染的抗生素，耳毒性是其副作用之一。耳毒性是由药物引起的医源性疾病，会影响内耳，影响耳蜗和/或前庭系统，并导致平衡和听力这两项重要功能出现改变。接受氨基糖苷类抗生素治疗的主要儿科群体是新生儿重症监护病房中患有严重感染的新生儿。
通过形态学——扫描电子显微镜（SEM）和功能学——畸变产物耳声发射（DPOAE）实验研究，验证每天4mg/kg和每12小时2.5mg/kg单剂量庆大霉素方案导致外毛细胞（ECC）出现的情况。
通过DPOAE对26只白化豚鼠在庆大霉素治疗前后进行评估。在肌肉注射药物后的设定时间处死豚鼠，以便使用扫描电子显微镜进行解剖分析。
对ECC功能状态的评估表明，所有豚鼠的DPOAE均保持正常。对SEM结果拍照后，在特定摄影视野中根据耳蜗基底转的ECC数量进行分析。
未观察到白化豚鼠在每12小时使用4mg/Kg/天和2.5mg/Kg/天，持续10天和14天后，其ECC功能出现损伤或改变。
