依卡倍特钠在大鼠反流性食管炎中的特征性病理表现及作用

Characteristic pathological findings and effects of ecabet sodium in rat reflux esophagitis.

作者信息

Asaoka Daisuke, Nagahara Akihito, Oguro Masako, Izumi Yuko, Kurosawa Akihiko, Osada Taro, Kawabe Masato, Hojo Mariko, Otaka Michiro, Watanabe Sumio

机构信息

Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.

出版信息

World J Gastroenterol. 2009 Jul 28;15(28):3480-5. doi: 10.3748/wjg.15.3480.

DOI:10.3748/wjg.15.3480

PMID:19630101

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2715972/

Abstract

AIM

To explore the pathological findings in the entire esophagus in rats with reflux esophagitis, and the effects of ecabet sodium (ES).

METHODS

A rat model of chronic acid reflux esophagitis was used. In the treatment group, ES was administered after surgery (n = 16). No drug was administered postoperatively to the esophagitis group (n = 9). Sham-operated rats were used as a control group (n = 5). Rats were sacrificed on day 7 after the operation. The epithelial thickness and leukocyte infiltration were examined in the upper, middle and lower areas of the esophagus. The survival rate, incidence of esophageal ulcer, and mean surface area and number of esophageal ulcers were determined in the esophagitis and ES groups. Esophageal histology was assessed in all three groups.

RESULTS

Leukocyte infiltration in the esophagitis group was 26.3 +/- 22.0 in the middle esophagus and 8.2 +/- 4.9 in the upper esophagus, which was significantly greater than that in the controls (1.3 +/- 1.1 and 1.4 +/- 1.0, respectively) (P < 0.05). The thickness of the epithelium in the esophagitis group was 210.8 +/- 47.7 microm in the lower esophagus and 204.2 +/- 60.1 microm in the middle esophagus, which was significantly greater than that in the controls (26.0 +/- 5.5 and 21.0 +/- 6.5 microm, respectively) (P < 0.05). The mean number of ulcers per animal in the ES group in the entire esophagus was 5.4 +/- 2.5, which was significantly less than that in the esophagitis group (9.0 +/- 3.5) (P < 0.05). The epithelial thickness in the ES group was 97.5 +/- 32.2 microm in the lower esophagus, which was decreased compared with that in the esophagitis group (210.8 +/- 47.7 microm) (P < 0.05).

CONCLUSION

Mucosal inflammation extended to the upper esophagus close to the hypopharynx. Our study suggested that ES may have a useful defensive role in reflux esophagitis.

摘要

目的

探讨反流性食管炎大鼠整个食管的病理变化以及依卡倍特钠（ES）的作用。

方法

采用大鼠慢性酸反流性食管炎模型。治疗组术后给予ES（n = 16）。食管炎组术后未给药（n = 9）。假手术大鼠作为对照组（n = 5）。术后第7天处死大鼠。检查食管上、中、下区域的上皮厚度和白细胞浸润情况。测定食管炎组和ES组的生存率、食管溃疡发生率、食管溃疡平均表面积和数量。对三组进行食管组织学评估。

结果

食管炎组食管中部白细胞浸润为26.3±22.0，食管上部为8.2±4.9，显著高于对照组（分别为1.3±1.1和1.4±1.0）（P < 0.05）。食管炎组食管下部上皮厚度为210.8±47.7μm，食管中部为204.2±60.1μm，显著高于对照组（分别为26.0±5.5和21.0±6.5μm）（P < 0.05）。ES组整个食管每只动物的溃疡平均数为5.4±2.5，显著少于食管炎组（9.0±3.5）（P < 0.05）。ES组食管下部上皮厚度为97.5±32.2μm，与食管炎组（210.8±47.7μm）相比有所降低（P < 0.05）。