Chamon Raiane C, Marques Lucas M, Timenetsky Jorge, da Costa Rachid Caio T C, Ferreira Rosana B R, de Oliveira Tamara L R, Glatthardt Thais, de Oliveira Moreira Lilian, Dos Santos Kátia R N
1Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, Brazil; 2Laboratório de Infecção Hospitalar, Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; 3Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Bahia, Brazil; 4Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil; 5Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; 6Laboratório de Bacteriologia e Imunologia Clínica, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Curr Genomics. 2020 Feb;21(2):128-137. doi: 10.2174/1389202921666200327105756.
isolates expressing the Panton-Valentine Leukocidin (PVL) have been related to a wide range of diseases. Recently, -positive community-associated methicillin-resistant belonging to USA1100 (ST30/CC30/ IV) lineage has emerged in Brazilian hospitals.
The aim of this work was to sequence the genome of a positive USA1100 Vancomycin-Intermediate-Resistant (VISA) isolate from Rio de Janeiro, Brazil.
The 13420 genome was sequenced using the HiSeq 2500 platform. The draft genome, plasmids annotation, and genome analysis were performed using RAST. Comparison of the relative gene expression of six isolates was performed by qRT-PCR.
The isolate presented the ϕPVL phage codifying for the H2b PVL protein isoform, and another prophage carrying a PVL variant named and -PV.2. The 13420 genome presented a high number of virulence determinants, such as genes codifying for serine-protease proteins, enterotoxins (), the immune evasion cluster (IEC), adhesion proteins, spermine/spermidine acetyltransferase gene (), superantigen-like proteins, as well as the operon. Point mutations at , and genes were detected. Moreover, the PVL mRNA relative expression of the 13420 isolate was five times higher than mRNA PVL levels of the USA300/ST8 reference strain.
We described for the first time the genome sequence of a VISA isolate harboring two -associated genes and other virulence factors that may improve the USA1100/ST30 lineage fitness and impact its pathogenicity and spreading at Brazilian hospitals.
表达杀白细胞素（PVL）的分离株与多种疾病有关。最近，属于USA1100（ST30/CC30/IV）谱系的耐甲氧西林金黄色葡萄球菌在巴西医院中出现。
本研究旨在对来自巴西里约热内卢的一株耐万古霉素中间型金黄色葡萄球菌（VISA）阳性USA1100分离株的基因组进行测序。
使用HiSeq 2500平台对13420菌株的基因组进行测序。使用RAST进行基因组草图绘制、质粒注释和基因组分析。通过qRT-PCR对6株金黄色葡萄球菌分离株的相对基因表达进行比较。
该分离株携带编码H2b PVL蛋白异构体的ϕPVL噬菌体，以及另一种携带名为ϕ-PVL.1和ϕ-PVL.2的PVL变体的原噬菌体。13420菌株的基因组具有大量毒力决定因素，如编码丝氨酸蛋白酶蛋白、肠毒素（SEs）、免疫逃避簇（IEC）、粘附蛋白、精胺/亚精胺乙酰转移酶基因（sat）、超抗原样蛋白的基因，以及lukS-PV-lukF-PV操纵子。在mecA、femA和femB基因中检测到点突变。此外，13420分离株的PVL mRNA相对表达量比USA300/ST8参考菌株的mRNA PVL水平高5倍。
我们首次描述了一株携带两个PVL相关基因和其他毒力因子的VISA分离株的基因组序列，这些基因和因子可能提高USA1100/ST30谱系的适应性，并影响其在巴西医院的致病性和传播。
