B Food Science Co. Ltd., Chita, Aichi 478-0046, Japan.
N Biotechnol. 2009 Oct 31;26(3-4):137-42. doi: 10.1016/j.nbt.2009.07.004. Epub 2009 Jul 22.
Cellobiose was enzymatically synthesized from starch using two phosphorylases. Under the presence of 1 M Pi inorganic phosphate), glucan phosphorylase converted 40% of glucose residues in the starch molecule into G1P (glucose-1-phosphate). By electrodialysis fitted with an ion exchange membrane having molecular weight cutoff of 100, Pi was effectively dialyzed out and G1P was recovered with 80% yield. G1P and glucose were incubated with cellobiose phosphorylase in the presence of magnesium acetate at an alkaline condition. Inorganic phosphate coformed with cellobiose was immediately removed as insoluble magnesium ammonium phosphate and 85% of added G1P was converted into cellobiose. On the whole, cellobiose was produced with 60% yield from G1P and, at least, 23.7% yield from starch.
纤维二糖是使用两种磷酸化酶从淀粉中酶促合成的。在 1 M Pi（无机磷酸盐）存在的情况下，葡聚糖磷酸化酶将淀粉分子中 40%的葡萄糖残基转化为 G1P（葡萄糖-1-磷酸）。通过配备有分子量截止值为 100 的离子交换膜的电渗析，有效地将 Pi 透析出来，并以 80%的产率回收 G1P。在碱性条件下，G1P 和葡萄糖与纤维二糖磷酸化酶一起孵育，在镁醋酸盐存在下。与纤维二糖形成的无机磷酸盐立即被去除为不溶性的镁铵磷酸盐，并且 85%的添加 G1P 转化为纤维二糖。总的来说，从 G1P 生产纤维二糖的产率为 60%，从淀粉生产纤维二糖的产率至少为 23.7%。
