Sun Shangshang, You Chun
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, People's Republic of China.
University of Chinese Academy of Sciences, 19A Yuquan Road, Shijingshan District, Beijing, 100049, People's Republic of China.
Synth Syst Biotechnol. 2021 Feb 13;6(1):23-31. doi: 10.1016/j.synbio.2021.01.004. eCollection 2021 Mar.
Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a mechanistic as well as a structural point of view, they are similar to glycoside hydrolases or glycosyltransferases. As the majority of DSPs show strict stereo- and regiospecificities, these enzymes were used to synthesize specific disaccharides. Currently, protein engineering of DSPs is pursued in different laboratories to broaden the donor and acceptor substrate specificities or improve the industrial particularity of naturally existing enzymes, to eventually generate a toolbox of new catalysts for glycoside synthesis. Herein we review the characteristics and classifications of reported DSPs and the glycoside products that they have been used to synthesize.
双糖磷酸化酶（DSPs）是一类具有显著潜力的碳水化合物活性酶，可将普通食糖生物催化转化为具有诱人特性的产物。它们是形成活性同型寡聚体的模块化酶。从机制和结构角度来看，它们与糖苷水解酶或糖基转移酶相似。由于大多数双糖磷酸化酶表现出严格的立体和区域特异性，这些酶被用于合成特定的二糖。目前，不同实验室正在进行双糖磷酸化酶的蛋白质工程研究，以拓宽供体和受体底物特异性，或改善天然存在酶的工业特性，最终生成用于糖苷合成的新型催化剂工具箱。在此，我们综述已报道的双糖磷酸化酶的特性和分类，以及它们已被用于合成的糖苷产物。
