Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Camobi-9, Prédio 20, Sala 4232, Santa Maria, RS, Brazil.
Vet Parasitol. 2009 Oct 28;165(1-2):47-50. doi: 10.1016/j.vetpar.2009.06.025. Epub 2009 Jun 25.
The aim of this study was to investigate the efficacy of diminazene aceturate in the control of the infection by Trypanosoma evansi in cats. Fourteen animals were infected with 10(8) trypomastigote forms each and six were used as negative control (group A). Seven of the infected cats were used as positive control (group B) and seven were treated with diminazene aceturate (3.5 mg kg(-1)) for 5 consecutive days (group C). Biochemical and hematological parameters were evaluated during the experiment. Blood with anticoagulant was collected at day 49 post-inoculation and preserved in ethanol for DNA extraction. Samples were analyzed using PCR T. evansi-specific to assess the effectiveness of treatment. The treatment with diminazene aceturate had an efficacy of 85.7%. Alanine aminotransferase, gamma-glutamyltransferase, urea, and creatinine values remained within the normal physiological range in the treated cats. Hemogram was normalized in all the cured animals. Therefore, the therapy used is effective in controlling T. evansi in cats.
本研究旨在探讨乙酰苯肼对感染伊氏锥虫的猫的疗效。将 14 只动物感染 10(8)个锥虫游离体，6 只作为阴性对照（A 组）。7 只感染猫作为阳性对照（B 组），7 只用乙酰苯肼（3.5mg/kg）连续治疗 5 天（C 组）。在实验过程中评估了生化和血液学参数。在接种后第 49 天采集抗凝血液并保存在乙醇中用于 DNA 提取。使用针对 T. evansi 的 PCR 分析样品，以评估治疗效果。乙酰苯肼的治疗效果为 85.7%。在治疗猫中，丙氨酸氨基转移酶、γ-谷氨酰转移酶、尿素和肌酐值仍在正常生理范围内。所有治愈的动物的血液常规都恢复正常。因此，该疗法可有效控制猫中的 T. evansi。
