Department of Physics & Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Acta Biomater. 2010 Jan;6(1):154-9. doi: 10.1016/j.actbio.2009.07.026. Epub 2009 Jul 23.
Poly(butylene succinate), a novel biodegradable aliphatic polyester with excellent processability and mechanical properties, was modified by O(2) or N(2) plasma immersion ion implantation (PIII). X-ray photoelectron spectroscopy and contact angle measurements were carried out to reveal the surface characteristics of the treated and control specimens. The in vitro effects of the materials on seeded osteoblasts were detected by cell viability assay, alkaline phosphatase activity test, and real-time polymerase chain reaction analysis. Plate counting was performed to investigate the antibacterial properties. Our results show that both PIII treatments significantly improve the hydrophilicity of PBSu, and CO and nitrogen groups (CNH and CNH(2)) can be detected on the PBSu after O(2) and N(2) PIII, respectively. The modified samples exhibit similar compatibility to osteoblasts, which is better than that of the control, but O(2) PIII and N(2) PIII produce different effects according to the osteogenic gene expressions of seeded osteoblasts on the materials. Moreover, the N(2) plasma-modified PBSu exhibits anti-infection effects against Staphylococcus aureus and Escherichia coli but no such effects can be achieved after O(2) PIII.
聚丁二酸丁二醇酯是一种新型的可生物降解脂肪族聚酯，具有优异的加工性能和机械性能。通过 O(2)或 N(2)等离子体浸没式离子注入（PIII）对其进行了改性。采用 X 射线光电子能谱和接触角测量来揭示处理和对照样品的表面特性。通过细胞活力测定、碱性磷酸酶活性测试和实时聚合酶链反应分析来检测材料对种骨细胞的体外影响。通过平板计数来研究抗菌性能。结果表明，两种 PIII 处理都显著提高了 PBSu 的亲水性，并且在 O(2)和 N(2) PIII 后，PBSu 上可以检测到 CO 和氮基团（CNH 和 CNH(2)）。改性样品对成骨细胞具有相似的相容性，优于对照样品，但 O(2) PIII 和 N(2) PIII 根据种骨细胞在材料上的成骨基因表达产生不同的效果。此外，N(2)等离子体改性的 PBSu 对金黄色葡萄球菌和大肠杆菌具有抗感染作用，但 O(2) PIII 则没有这种效果。
