Lindner Robert, Naim Hassan Y
Department of Cell Biology, Hannover Medical School, Hannover, Germany.
Exp Cell Res. 2009 Oct 15;315(17):2871-8. doi: 10.1016/j.yexcr.2009.07.020. Epub 2009 Jul 23.
Our current view on biological membranes has gradually evolved from the influential fluid mosaic model of the early 1970s to a distinctively more complex picture. Biological membranes are now assumed to encompass multiple membrane domains and a plethora of protein-lipid and protein-protein interactions that compartmentalize and temporarily order what has originally been envisioned to be mostly random. In this minireview, we will first highlight some structural principles that govern membrane domain formation and permit a classification of membrane domains. We will then focus on the still controversial issue of lipid-based membrane domains, or lipid rafts, and discuss recent advances in detecting these enigmatic structures in living cells. Finally we will evaluate biochemical approaches to characterize lipid rafts and discuss their contribution to the emerging topic of lipid raft diversity.
我们目前对生物膜的看法已逐渐从20世纪70年代初颇具影响力的流体镶嵌模型演变为一幅明显更为复杂的图景。如今，人们认为生物膜包含多个膜结构域以及大量的蛋白质 - 脂质和蛋白质 - 蛋白质相互作用，这些相互作用将原本设想为大多随机的成分进行分隔并使其暂时有序排列。在这篇小型综述中，我们将首先强调一些支配膜结构域形成并允许对膜结构域进行分类的结构原理。然后，我们将聚焦于基于脂质的膜结构域（即脂筏）这一仍存在争议的问题，并讨论在活细胞中检测这些神秘结构的最新进展。最后，我们将评估用于表征脂筏的生化方法，并讨论它们对脂筏多样性这一新兴主题的贡献。
