Bailer S M, Haas J
Max-von-Pettenkofer Institut, Ludwig-Maximilians-Universität München, Muenchen, Germany.
Curr Opin Microbiol. 2009 Aug;12(4):453-9. doi: 10.1016/j.mib.2009.06.004. Epub 2009 Jul 24.
Genome-scale screens for intraviral and virus-host protein interactions and the analysis of literature-curated datasets are able to provide a novel, comprehensive perspective of viruses, and virus-infected cells. Until now, large-scale interaction screens were predominantly performed with the yeast-two-hybrid (Y2H) system; however, alternative high-throughput technologies detecting binary protein interactions or protein complexes have been developed. Although many of the previous studies suffer from a rather poor validation of the results and few biological implications, these technologies potentially lead to a plethora of novel hypotheses. Here, we will give an overview of current approaches and their technical limitations, present recent examples and novel developments.
针对病毒内和病毒-宿主蛋白相互作用的全基因组规模筛选以及对文献整理数据集的分析，能够为病毒和病毒感染细胞提供全新的、全面的视角。到目前为止，大规模相互作用筛选主要是利用酵母双杂交（Y2H）系统进行的；然而，已经开发出了检测二元蛋白相互作用或蛋白复合物的其他高通量技术。尽管之前的许多研究在结果验证方面相当不足，且生物学意义有限，但这些技术有可能产生大量新的假设。在这里，我们将概述当前的方法及其技术局限性，展示近期的实例和新进展。
