用于果蝇差异谱系分析的双斑生成器。
The twin spot generator for differential Drosophila lineage analysis.
作者信息
Griffin Ruth, Sustar Anne, Bonvin Marianne, Binari Richard, del Valle Rodriguez Alberto, Hohl Amber M, Bateman Jack R, Villalta Christians, Heffern Elleard, Grunwald Didier, Bakal Chris, Desplan Claude, Schubiger Gerold, Wu C-ting, Perrimon Norbert
机构信息
Commissariat à l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France.
出版信息
Nat Methods. 2009 Aug;6(8):600-2. doi: 10.1038/nmeth.1349. Epub 2009 Jul 26.
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.
在黑腹果蝇中，广泛使用的基于有丝分裂重组的策略产生的嵌合体果蝇，在分裂后只有一个子细胞具有阳性读数。为了对两个子细胞进行差异标记，我们开发了双斑生成器（TSG）技术，该技术通过有丝分裂重组产生绿色和红色双斑，在第一次细胞分裂后作为单个细胞可检测到。我们提出TSG在谱系和遗传嵌合体研究中的广泛应用。
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