Huang Hui-lian, Zhang Jin-qiang, Chena Guang-tong, Lu Zhi-qiang, Sha Na, Guo De-an
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, PR China.
Nat Prod Commun. 2009 Jun;4(6):825-30.
Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 x 4.6 mm, 5 microm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively.
氧化白藜芦醇（反式-2,4,3',5'-四羟基二苯乙烯，OXY）和白藜芦醇（反式-3,5,4'-三羟基二苯乙烯，RES）是中药菝葜的两种最重要成分。建立了一种反相高效液相色谱法，用于测定大鼠口服菝葜提取物后胆汁和尿液中的OXY和RES。生物样品采用高效液相色谱法在Aglient Zorbax SB-C18柱（250×4.6 mm，5μm）上进行分析，检测波长为320 nm，流速为1.0 mL/min。该方法测定准确、重现性好。胆汁样品中OXY和RES的累积排泄率分别为0.29%和0.97%，尿液样品中分别为0.84%和0.65%。
