采用液相色谱/串联质谱法鉴定大鼠尿液和胆汁中氧化白藜芦醇的七种代谢物。

Identification of seven metabolites of oxyresveratrol in rat urine and bile using liquid chromatography/tandem mass spectrometry.

作者信息

Huang Huilian, Chen Guangtong, Lu Zhiqiang, Zhang Jinqiang, Guo De-An

机构信息

Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 199 Guo Shoujing Road, Zhangjiang, Shanghai, People's Republic of China.

出版信息

Biomed Chromatogr. 2010 Apr;24(4):426-32. doi: 10.1002/bmc.1309.

DOI:10.1002/bmc.1309

PMID:19662623

Abstract

Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene) is a major compound isolated from Smilax china, a Chinese herbal medicine. The rat urine and bile samples were pretreated by solid-phase extraction method after oral administration at a dose of 100 mg/kg of oxyresveratrol. Seven metabolites were identified by LC-MS/MS method with electrospray ionization in negative ion mode. The results indicated that main metabolites of oxyresveratrol were monoglucuronided and monosulfated oxyresveratrol. Based on the results, the metabolic pathway of oxyresveratrol in rat urine and bile was proposed.

摘要

氧化白藜芦醇（反式-2,4,3',5'-四羟基二苯乙烯）是从中国草药菝葜中分离出的一种主要化合物。以100mg/kg的剂量口服氧化白藜芦醇后，采用固相萃取法对大鼠尿液和胆汁样本进行预处理。通过液相色谱-串联质谱法在负离子模式下的电喷雾电离鉴定出7种代谢产物。结果表明，氧化白藜芦醇的主要代谢产物是单葡萄糖醛酸化和单硫酸化的氧化白藜芦醇。基于这些结果，提出了氧化白藜芦醇在大鼠尿液和胆汁中的代谢途径。