Li Lili, Ye YanRui, Pan Li, Zhu Yi, Zheng SuiPing, Lin Ying
South China University of Technology, Guangzhou, PR China.
Biochem Biophys Res Commun. 2009 Oct 2;387(4):778-83. doi: 10.1016/j.bbrc.2009.07.113. Epub 2009 Jul 25.
Trehalose and glycerol have been implicated as potential stress protectants that accumulate in yeasts during various stress conditions. We investigated the levels of glycerol and trehalose and the expression profiles of genes involved in their metabolism to determine their involvement in the response of Saccharomyces cerevisiae XQ1 to thermal, sorbitol and ethanol stresses. The results showed that the genes involved in the synthesis and degradation of trehalose and glycerol were stress induced, and that trehalose and glycerol were synthesized simultaneously during the initial stages (a sensitive response period) of diverse stress treatments. Trehalose accumulated markedly under heat treatment, but not under sorbitol or ethanol stress, whereas glycerol accumulated strikingly under sorbitol stress conditions. Interestingly, extracellular trehalose seemed to be involved in protecting cells from damage under unfavorable conditions. Moreover, our results suggest that the stress-activated futile ATP cycles of trehalose and glycerol turnover are of general importance during cellular stress adaptation.
海藻糖和甘油被认为是潜在的应激保护剂，在各种应激条件下会在酵母中积累。我们研究了甘油和海藻糖的水平以及参与其代谢的基因的表达谱，以确定它们在酿酒酵母XQ1对热、山梨醇和乙醇应激反应中的作用。结果表明，参与海藻糖和甘油合成与降解的基因受到应激诱导，并且在不同应激处理的初始阶段（敏感反应期），海藻糖和甘油同时合成。海藻糖在热处理下显著积累，但在山梨醇或乙醇应激下不积累，而甘油在山梨醇应激条件下显著积累。有趣的是，细胞外海藻糖似乎在不利条件下参与保护细胞免受损伤。此外，我们的结果表明，海藻糖和甘油周转的应激激活无效ATP循环在细胞应激适应过程中具有普遍重要性。
