Okoko Tebekeme
Biochemistry Division, Department of Chemical Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.
Food Chem Toxicol. 2009 Oct;47(10):2620-3. doi: 10.1016/j.fct.2009.07.023. Epub 2009 Jul 25.
Garcinia kola Heckel--a tropical plant which grows in moist forest, has found wide applications in traditional medicine especially in the West and Central African sub-region. The seeds have been demonstrated to possess numerous bioactivities but research is highly limited on the link between its fractions and the bioactivities. In this work, the methanolic extract of Garcinia kola seeds was subjected to silica gel column chromatography into five fractions ME1-ME5 and the free radical scavenging activities and antioxidant potentials were determined for each fraction using various in vitro models. The ME4 fraction possessed the greatest activities. It was also demonstrated that the ME4 fraction strongly inhibited nitric oxide production in lipopolysaccharide activated macrophage U937 cells. Chromatographic fractionation and spectroscopic analysis of the ME4 fraction revealed the presence of four compounds namely garcinia biflavonoids GB1 and GB2, garcinal and garcinoic acid. These findings show that these four compounds are partly responsible for the great antioxidant potential of Garcinia kola seeds. This gives further evidence to the nutraceutical and pharmaceutical potential of Garcinia kola.
可乐果（Garcinia kola Heckel）是一种生长在潮湿森林中的热带植物，在传统医学中有着广泛应用，尤其是在西非和中非次区域。已证明其种子具有多种生物活性，但关于其各组分与生物活性之间联系的研究非常有限。在这项研究中，可乐果种子的甲醇提取物经硅胶柱色谱法分离为五个组分ME1 - ME5，并使用各种体外模型测定了每个组分的自由基清除活性和抗氧化潜力。ME4组分具有最强的活性。还证明ME4组分能强烈抑制脂多糖激活的巨噬细胞U937细胞中一氧化氮的产生。对ME4组分进行色谱分离和光谱分析，发现其中存在四种化合物，即藤黄双黄酮GB1和GB2、藤黄灵和藤黄酸。这些发现表明，这四种化合物是可乐果种子具有强大抗氧化潜力的部分原因。这进一步证明了可乐果在营养保健品和制药方面的潜力。
