Silvestre Ricardo, Santarém Nuno, Teixeira Lúcia, Cunha Joana, Schallig Henk, Cordeiro-da-Silva Anabela
Parasite Disease Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal.
Am J Trop Med Hyg. 2009 Aug;81(2):202-8.
The sensitivities and specificities of IgG-ELISA and IgG flow cytometry based techniques using different Leishmania species were determined using sera collected from 40 cutaneous or visceral leishmaniasis patients. The flow cytometry technique, using promastigote parasite forms, performed better than total soluble extract IgG-ELISA. At the species level, the use of Leishmania amazonensis and Leishmania major as antigens in enzyme linked immunosorbent assay (ELISA) decreased the overall sensitivity. To assess the specificity of these tests, sera from malaria, toxoplasmosis, amoebiasis, schistosomiasis, and leprosy patients were used. We also included sera from Leishmania non-infected endemic individuals. The cutaneous species displayed a decreased specificity in both assays. Although more sensitive, flow cytometry using promastigote parasite forms generally presented lower levels of specificity when compared with total extract of IgG-ELISA. Overall, the results of the study show the potential of IgG flow cytometry for the diagnosis of leishmaniasis. Although highly sensitive, a refinement of the flow cytometry method should be performed to improve the overall specificity.
使用从40名皮肤或内脏利什曼病患者采集的血清，测定了基于IgG-ELISA和IgG流式细胞术的技术对不同利什曼原虫物种的敏感性和特异性。使用前鞭毛体寄生虫形式的流式细胞术技术比总可溶性提取物IgG-ELISA表现更好。在物种水平上，在酶联免疫吸附测定（ELISA）中使用亚马逊利什曼原虫和硕大利什曼原虫作为抗原会降低总体敏感性。为了评估这些检测的特异性，使用了疟疾、弓形虫病、阿米巴病、血吸虫病和麻风病患者的血清。我们还纳入了未感染利什曼原虫的流行地区个体的血清。皮肤利什曼原虫物种在两种检测中均表现出特异性降低。尽管更敏感，但与IgG-ELISA总提取物相比，使用前鞭毛体寄生虫形式的流式细胞术通常表现出较低的特异性水平。总体而言，研究结果显示了IgG流式细胞术在利什曼病诊断中的潜力。尽管高度敏感，但应改进流式细胞术方法以提高总体特异性。
