Price R E, Templeton J W, Adams L G
Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Vet Immunol Immunopathol. 1990 Dec;26(4):353-65. doi: 10.1016/0165-2427(90)90119-d.
Transposon mutants offer a unique way to evaluate the role of lipopolysaccharide (LPS) by producing a theoretical single-gene difference between the original strain and the transposon mutant strain. Comparative survival of Brucella abortus smooth strain 2308, rough RB51, smooth strain 19, and two transposon mutant strains (rough strain 2308::Tn5 Lac Z [m106] and rough strain 19::Tn5 Lac Z [m3], was tested in restrictive bovine mammary macrophages that were able to effectively reduce the percentage of intracellular bacterial survival and permissive bovine mammary macrophages that were unable to control the intracellular replication of B. abortus. The theoretical single-gene difference between strain 19 and strain 19::Tn5 lac Z [m3] and between smooth virulent strain 2308 and rough transposon mutant 2308::Tn5 lacZ [m106] is likely related to differences in LPS content or structure. Significant (P less than 0.05) reduction in the survival of rough strain 19::Tn5 Lac Z [m3] with no significant reduction in the rough transposon mutant strain 2308::Tn5 lacZ [m106] indicated that at least one factor other than LPS contributes to the intracellular survival of B. abortus in bovine macrophages.
转座子突变体提供了一种独特的方法来评估脂多糖（LPS）的作用，通过在原始菌株和转座子突变体菌株之间产生理论上的单基因差异。对流产布鲁氏菌光滑菌株2308、粗糙型RB51、光滑菌株19以及两个转座子突变体菌株（粗糙菌株2308::Tn5 Lac Z [m106]和粗糙菌株19::Tn5 Lac Z [m3]）在具有有效降低细胞内细菌存活百分比的限制性牛乳腺巨噬细胞和无法控制流产布鲁氏菌细胞内复制的允许性牛乳腺巨噬细胞中的比较存活率进行了测试。菌株19与菌株19::Tn5 lac Z [m3]之间以及光滑有毒菌株2308与粗糙转座子突变体2308::Tn5 lacZ [m106]之间的理论单基因差异可能与LPS含量或结构的差异有关。粗糙菌株19::Tn5 Lac Z [m3]的存活率显著（P小于0.05）降低，而粗糙转座子突变体菌株2308::Tn5 lacZ [m106]的存活率没有显著降低，这表明除LPS外至少还有一个因素有助于流产布鲁氏菌在牛巨噬细胞中的细胞内存活。
