Centro Interdipartimentale di Ricerca per le Scienze Ambientali, Università di Bologna, Via S'Alberto 163, 48100 Ravenna, Italy.
Toxicon. 2010 Feb-Mar;55(2-3):211-20. doi: 10.1016/j.toxicon.2009.07.019. Epub 2009 Jul 26.
Massive blooms of the benthic dinoflagellate Ostreopsis ovata Fukuyo have recently occurred along the whole Italian coastlines, both Tyrrhenian and Adriatic, resulting sometimes in benthonic biocenosis sufferings and, occasionally, in human health problems. In this work, two strains of O. ovata collected in 2006 along the Adriatic and Tyrrhenian coastlines and grown in culture were studied to characterize their growth and toxin profile. The two strains showed different cell volumes, the Adriatic strain being nearly twice bigger than the Tyrrhenian, but they had similar slow growth rates. Liquid chromatography-mass spectrometry (LC-MS) analyses indicated that both strains produce putative palytoxin (pPLTX) and ovatoxin-a (OVTX-a), a palytoxin-like compound presenting 2 oxygen atoms less than palytoxin. Toxin content was determined at the end of the stationary and exponential growth phases and reached the highest value in the Adriatic strain at the end of the stationary phase, with concentrations of 353.3 microg l(-1) for OVTX-a and 30.4 microg l(-1) for pPLTX. Toxin released in the growth medium was also measured and resulted to be the highest at the end of the stationary phase, suggesting that a long lasting bloom could enhance the toxin content in the water and cause toxic effects in people inhaling the aerosol.
底栖腰鞭毛虫夜光藻的大量繁殖最近发生在整个意大利海岸线，无论是第勒尼安海还是亚得里亚海，这导致底栖生物群落有时遭受痛苦，偶尔还会导致人类健康问题。在这项工作中，研究了 2006 年在亚得里亚海和第勒尼安海沿岸采集并在培养中生长的两种夜光藻，以表征它们的生长和毒素特征。这两种菌株的细胞体积不同，亚得里亚海菌株的体积几乎是第勒尼安海菌株的两倍，但它们的生长速度相似。液相色谱-质谱（LC-MS）分析表明，两种菌株都产生可能的棕榈毒素（pPLTX）和卵形毒素-a（OVTX-a），这是一种比棕榈毒素少两个氧原子的棕榈毒素类似物。在静止和指数生长阶段结束时测定了毒素含量，在静止阶段结束时，亚得里亚海菌株的含量最高，OVTX-a 的浓度为 353.3μg l（-1），pPLTX 的浓度为 30.4μg l（-1）。还测量了生长培养基中释放的毒素，结果表明在静止阶段结束时最高，这表明长时间的水华可能会增加水中的毒素含量，并导致吸入气溶胶的人产生毒性作用。
