Department of Chemistry of Natural Products, University of Napoli Federico II, via D. Montesano 49, Napoli 80131, Italy.
Chem Res Toxicol. 2012 Jun 18;25(6):1243-52. doi: 10.1021/tx300085e. Epub 2012 Apr 26.
Currently, the benthic dinoflagellate Ostreopsis cf. ovata represents a serious concern to human health in the whole Mediterranean basin due to the production of palytoxin congeners, a putative palytoxin and ovatoxins (ovatoxin-a, -b, -c, -d/-e), listed among the most potent marine toxins. High resolution liquid chromatography-mass spectrometry (HR LC-MS) based investigation of a North Western Adriatic strain of Ostreopsis cf. ovata collected at Portonovo (Italy) in 2008 is reported herein. Toxin profile was different from those previously reported for other O. cf. ovata, both qualitatively and quantitatively. For the first time, ovatoxin-a did not dominate the toxin profile, and a new palytoxin congener, here named ovatoxin-f, was detected. Ovatoxin-f and its elemental formula present C(2)H(4) more than ovatoxin-a. HR CID MS(n) experiments allowed us to restrict structural differences between ovatoxin-a and -f to the region between C-95 and C-102, a region not previously been described to be modified in other palytoxins. Ovatoxin-f represents the major component of the toxin profile of the analyzed strain accounting for 50% of the total toxin content, while ovatoxin-a, the dominant toxin in most of the Mediterranean O. cf. ovata strains we have analyzed so far, is the second major component of the toxin profile (23%). Thus, the presence of ovatoxin-f should be taken into account when monitoring programs for palytoxin-like compounds in microalgae and/or seawater are carried out.
目前，底栖甲藻腰鞭毛虫 Ostreopsis cf. ovata 由于产生类似肉毒毒素的物质、一种假定的肉毒毒素和卵毒素（卵毒素-a、-b、-c、-d/-e），而成为整个地中海盆地人类健康的严重关切。本文报道了 2008 年在意大利波托诺沃采集的西北亚得里亚海 Ostreopsis cf. ovata 菌株的基于高分辨率液相色谱-质谱（HR LC-MS）的调查。毒素特征在定性和定量上都与以前报告的其他 O. cf. ovata 不同。首次发现，卵毒素-a 并未主导毒素特征，并且检测到一种新的肉毒毒素类似物，命名为卵毒素-f。卵毒素-f 和其元素式比卵毒素-a 多 C(2)H(4)。HR CID MS(n) 实验使我们能够将卵毒素-a 和 -f 之间的结构差异限制在 C-95 和 C-102 之间的区域，该区域以前在其他肉毒毒素中未被描述为修饰过的区域。卵毒素-f 是分析菌株毒素特征的主要成分，占总毒素含量的 50%，而在我们迄今为止分析的大多数地中海 O. cf. ovata 菌株中占主导地位的毒素卵毒素-a 是毒素特征的第二大成分（23%）。因此，在进行微藻和/或海水中类似肉毒毒素化合物的监测计划时，应考虑到卵毒素-f 的存在。
