Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy.
Rapid Commun Mass Spectrom. 2010 Sep;24(18):2735-44. doi: 10.1002/rcm.4696.
Over the past decades, Italian coastlines have been plagued by recurring presence of the benthic dinoflagellate Ostreopsis ovata. Such an alga has caused severe sanitary emergencies and economic losses due to its production of palytoxin-like compounds. Previous studies have confirmed the presence of ovatoxin-a (OVTX-a) as the major toxin of the algal toxin profile together with small amounts of putative palytoxin (PLTX). In our ongoing research on O. ovata toxins we report herein on in-depth investigation of an O. ovata culture carried out by high-resolution (HR) liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry (MS(2)). Particularly, the presence of putative PLTX and OVTX-a was confirmed and the occurrence in the extract of four new palytoxin-like compounds, OVTX-b, -c, -d, and -e, was highlighted. Elemental formulae have been assigned to the new ovatoxins and information has been gained about their structural features. A quantitative study of the O. ovata culture extract indicated that the whole of the new ovatoxins represents about 46% of the total toxin content and, thus, their presence has to be taken into account when LC/MS-based monitoring programs of either plankton or contaminated seafood are carried out.
在过去的几十年里，意大利的海岸线一直受到底栖甲藻夜光藻的频繁侵袭。由于其产生类似 palytoxin 的化合物，这种藻类已经引发了严重的卫生紧急情况和经济损失。先前的研究已经证实，ovatoxin-a (OVTX-a) 是藻类毒素谱中的主要毒素，同时还有少量假定的 palytoxin (PLTX)。在我们对夜光藻毒素的持续研究中，我们在此报告了通过高分辨率（HR）液相色谱/质谱（LC/MS）和串联质谱（MS(2)）对一种夜光藻培养物进行的深入调查。特别是，确认了假定的 PLTX 和 OVTX-a 的存在，并强调了在提取物中存在四种新的 palytoxin 类似物，即 OVTX-b、-c、-d 和 -e。已经为新的 ovatoxins 分配了元素公式，并获得了有关其结构特征的信息。对夜光藻培养物提取物的定量研究表明，所有新的 ovatoxins 约占总毒素含量的 46%，因此，在进行浮游生物或受污染海鲜的基于 LC/MS 的监测计划时，必须考虑到它们的存在。
