根癌土壤杆菌T37分离株的pTiT37质粒DNA中插入序列元件IS427的鉴定。

Identification of insertion sequence element IS427 in pTiT37 plasmid DNA of an Agrobacterium tumefaciens T37 isolate.

作者信息

De Meirsman C, Croes C, Desair J, Verreth C, Van Gool A, Vanderleyden J

机构信息

F. A. Janssens Memorial Laboratory for Genetics, University of Leuven,Heverlee, Belgium.

出版信息

Plasmid. 1989 Mar;21(2):129-37. doi: 10.1016/0147-619x(89)90056-5.

DOI:10.1016/0147-619x(89)90056-5

PMID:2544912

Abstract

The isolation and characterization of an insertion sequence (IS) element, IS427, from Agrobacterium tumefaciens T37 is described. IS427 is present in three nonidentical copies on the pTiT37 plasmid. The copy that was isolated through transposition on the entrapment vector pUCD800 contains at its ends a 16-bp imperfect inverted repeat and generates a 2-bp duplication of the target DNA. IS427 does not show homology with previously characterized IS elements of A. tumefaciens, based on hybridization experiments and/or sequence comparison.

摘要

本文描述了从根癌农杆菌T37中分离和鉴定一个插入序列（IS）元件IS427的过程。IS427以三个不同的拷贝形式存在于pTiT37质粒上。通过在捕获载体pUCD800上转座而分离出的拷贝在其末端含有一个16bp的不完全反向重复序列，并导致靶DNA产生2bp的重复。基于杂交实验和/或序列比较，IS427与根癌农杆菌先前鉴定的IS元件没有同源性。