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Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA.根癌土壤杆菌pTi T37 Ti质粒T-DNA的tmr基因座的核苷酸序列。
Nucleic Acids Res. 1984 Jun 11;12(11):4665-77. doi: 10.1093/nar/12.11.4665.
2
Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37.根癌土壤杆菌T37菌株tzs基因的克隆及核苷酸序列分析
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3
Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli.根癌土壤杆菌章鱼碱型Ti质粒毒力基因virG的核苷酸序列：virG与大肠杆菌的调控基因ompR、phoB和dye之间存在显著同源性。
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本文引用的文献

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Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA.冠瘿瘤中的细胞分裂素/生长素平衡受 T-DNA 中特定基因座的调节。
Proc Natl Acad Sci U S A. 1983 Jan;80(2):407-11. doi: 10.1073/pnas.80.2.407.
2
A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell.冠瘿瘤细胞自主生长的生理基础
Proc Natl Acad Sci U S A. 1958 Apr;44(4):344-9. doi: 10.1073/pnas.44.4.344.
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EMBO J. 1983;2(6):987-95. doi: 10.1002/j.1460-2075.1983.tb01532.x.
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Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.在毛叶冠瘿碱型根瘤中鉴定 TL-DNA 转录本的功能
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The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria.Ti质粒中T区域的保守部分在细菌中表达四种蛋白质。
EMBO J. 1983;2(3):403-9. doi: 10.1002/j.1460-2075.1983.tb01437.x.
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Multiple transcripts of T-DNA detected in nopaline crown gall tumors.在胭脂碱型冠瘿瘤中检测到T-DNA的多个转录本。
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Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.根癌土壤杆菌Ti质粒编码的章鱼碱合酶基因的核苷酸序列和转录图谱。
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The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean.大豆中编码1,5-二磷酸核酮糖羧化酶小亚基的多基因家族一个成员的核苷酸序列、表达及进化
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10
Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of higher plant cells.基因在农杆菌致瘤质粒T-DNA上的位点特异性插入：一种高等植物细胞基因工程方法。
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根癌土壤杆菌pTi T37 Ti质粒T-DNA的tmr基因座的核苷酸序列。

Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA.

作者信息

Goldberg S B, Flick J S, Rogers S G

出版信息

Nucleic Acids Res. 1984 Jun 11;12(11):4665-77. doi: 10.1093/nar/12.11.4665.

DOI:10.1093/nar/12.11.4665
PMID:6330678
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318866/
Abstract

The nucleotide sequence of the tmr locus from the nopaline-type pTi T37 plasmid of Agrobacterium tumefaciens was determined. Examination of this sequence allowed us to identify an open reading frame of 720 nucleotides capable of encoding a protein with a derived molecular weight of 27025 d. Comparison of the pTi T37 tmr sequence with the published sequence of the pTi Ach5 tmr locus shows over 88% homology in the 240 bases 5' to the translational initiation codon and over 91% homology in the coding sequences. The 3' nontranslated regions show less than 50% homology as expected for the 3' regions of divergent related genes. The possible significance of areas of conserved sequences, particularly in the 5' regulatory regions, is discussed.

摘要

测定了根癌土壤杆菌胭脂碱型pTi T37质粒tmr基因座的核苷酸序列。对该序列的研究使我们能够鉴定出一个720个核苷酸的开放阅读框，它能够编码一个推导分子量为27025 d的蛋白质。将pTi T37的tmr序列与已发表的pTi Ach5 tmr基因座序列进行比较，发现在翻译起始密码子5'端的240个碱基中同源性超过88%，在编码序列中同源性超过91%。如预期的那样，3'非翻译区的同源性低于50%，这是不同相关基因3'区域的特征。文中讨论了保守序列区域，特别是5'调控区的可能意义。