根癌土壤杆菌pTi T37 Ti质粒T-DNA的tmr基因座的核苷酸序列。
Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA.
作者信息
Goldberg S B, Flick J S, Rogers S G
出版信息
Nucleic Acids Res. 1984 Jun 11;12(11):4665-77. doi: 10.1093/nar/12.11.4665.
Abstract
The nucleotide sequence of the tmr locus from the nopaline-type pTi T37 plasmid of Agrobacterium tumefaciens was determined. Examination of this sequence allowed us to identify an open reading frame of 720 nucleotides capable of encoding a protein with a derived molecular weight of 27025 d. Comparison of the pTi T37 tmr sequence with the published sequence of the pTi Ach5 tmr locus shows over 88% homology in the 240 bases 5' to the translational initiation codon and over 91% homology in the coding sequences. The 3' nontranslated regions show less than 50% homology as expected for the 3' regions of divergent related genes. The possible significance of areas of conserved sequences, particularly in the 5' regulatory regions, is discussed.
摘要
测定了根癌土壤杆菌胭脂碱型pTi T37质粒tmr基因座的核苷酸序列。对该序列的研究使我们能够鉴定出一个720个核苷酸的开放阅读框，它能够编码一个推导分子量为27025 d的蛋白质。将pTi T37的tmr序列与已发表的pTi Ach5 tmr基因座序列进行比较，发现在翻译起始密码子5'端的240个碱基中同源性超过88%，在编码序列中同源性超过91%。如预期的那样，3'非翻译区的同源性低于50%，这是不同相关基因3'区域的特征。文中讨论了保守序列区域，特别是5'调控区的可能意义。
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