Tang Li-Ying, Wang Zhu-Ju, Wu Qiu-Ping, He Yan, Huang Lu-Qi
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2008 Feb;33(4):366-8.
To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.
The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.
A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).
The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
建立决明子中红镰霉素龙胆二糖苷含量的测定方法。
采用Diamonsil C18（4.6 mm×250 mm，5μm）色谱柱，以乙腈 - 四氢呋喃 - 1%乙酸（18∶3∶79）为流动相，流速为1 mL·min⁻¹，柱温40℃，检测波长278 nm。
红镰霉素龙胆二糖苷在0.1 - 0.5μg范围内线性关系良好，r = 0.999 9。平均回收率为101.1%，RSD为2.23%（n = 5）。
该方法简便、快速、灵敏、准确、可靠、重复性好，可用于决明子的质量控制。
