Hao Jie, Zhu WanWan, Sheng Chao, Yu Yang, Zhou Qi
State Key Laboratory of Reproductive Biology, Institute of Zoology, Beijing, 100101, China.
Sci China C Life Sci. 2009 Jul;52(7):599-602. doi: 10.1007/s11427-009-0096-2. Epub 2009 Jul 30.
Pluripotent stem cells derived from somatic cells through such processes as nuclear transfer or induced pluripotent stem (iPS) cells present an important model for biomedical research and provide potential resources for cell replacement therapies. However, the overall efficiency of the conversional nuclear transfer is very low and the safety issue remains a major concern for iPS cells. Embryonic stem cells (ESCs) generated from parthenogenetic embryos are one attractive alternative as a source of histocompatible cells and tissues for cell therapy. Recent studies on human parthenogenetic embryonic stem cells (hPG ESCs) have revealed that these ESCs are very similar to the hESCs derived from IVF or in vivo produced blastocysts in gene expression and other characteristics, but full differentiation and development potential of these hPG ESCs have to be further investigated before clinical research and therapeutic interventions. To generate various pluripotent stem cells, diverse reprogramming techniques and approaches will be developed and integrated. This may help elucidate the fundamental mechanisms underlying reprogramming and stem cell biology, and ultimately benefit cell therapy and regenerative medicine.
通过核移植或诱导多能干细胞（iPS细胞）等过程从体细胞衍生而来的多能干细胞，为生物医学研究提供了重要模型，并为细胞替代疗法提供了潜在资源。然而，转化核移植的整体效率非常低，安全问题仍是iPS细胞的主要关注点。孤雌生殖胚胎产生的胚胎干细胞（ESCs）作为细胞治疗中组织相容性细胞和组织的来源，是一种有吸引力的替代方案。最近关于人类孤雌生殖胚胎干细胞（hPG ESCs）的研究表明，这些ESCs在基因表达和其他特征方面与源自体外受精或体内产生的囊胚的hESCs非常相似，但在进行临床研究和治疗干预之前，这些hPG ESCs的完全分化和发育潜力还有待进一步研究。为了产生各种多能干细胞，将开发和整合多种重编程技术和方法。这可能有助于阐明重编程和干细胞生物学的基本机制，并最终使细胞治疗和再生医学受益。
