通过优化培养条件，快速将人 ESC 转化为具有小鼠 ESC 样多能性的状态。
Rapid conversion of human ESCs into mouse ESC-like pluripotent state by optimizing culture conditions.
机构信息
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
出版信息
Protein Cell. 2012 Jan;3(1):71-9. doi: 10.1007/s13238-012-2007-8. Epub 2012 Jan 21.
Abstract
The pluripotent state between human and mouse embryonic stem cells is different. Pluripotent state of human embryonic stem cells (ESCs) is believed to be primed and is similar with that of mouse epiblast stem cells (EpiSCs), which is different from the naïve state of mouse ESCs. Human ESCs could be converted into a naïve state through exogenous expression of defined transcription factors (Hanna et al., 2010). Here we report a rapid conversion of human ESCs to mouse ESC-like naïve states only by modifying the culture conditions. These converted human ESCs, which we called mhESCs (mouse ESC-like human ESCs), have normal karyotype, allow single cell passage, exhibit domed morphology like mouse ESCs and express some pluripotent markers similar with mouse ESCs. Thus the rapid conversion established a naïve pluripotency in human ESCs like mouse ESCs, and provided a new model to study the regulation of pluripotency.
摘要
人和小鼠胚胎干细胞的多能状态不同。人类胚胎干细胞（ESCs）的多能状态被认为是预先形成的，类似于小鼠胚胎外胚层干细胞（EpiSCs），与小鼠 ESCs 的原始状态不同。通过外源性表达定义的转录因子，人类 ESCs 可以转化为原始状态（Hanna 等人，2010）。在这里，我们仅通过修改培养条件，就报告了一种将人类 ESCs 快速转化为具有小鼠 ESC 样原始状态的方法。我们将这些转化后的人类 ESCs 称为 mhESCs（具有小鼠 ESC 样特性的人类 ESCs），它们具有正常的核型，允许单细胞传代，呈现出类似于小鼠 ESCs 的穹顶形态，并表达一些与小鼠 ESCs 相似的多能标记物。因此，这种快速转化在人类 ESCs 中建立了类似于小鼠 ESCs 的原始多能性，并为研究多能性的调控提供了一个新模型。
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