Kim Nam Kuk, Lee Seung Hwan, Cho Yong Min, Son Eun Suk, Kim Kyung Yun, Lee Chang Soo, Yoon Duhak, Im Seok Ki, Oh Sung Jong, Park Eung Woo
National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.
BMB Rep. 2009 Jul 31;42(7):433-8. doi: 10.5483/bmbrep.2009.42.7.433.
The objective of this study was to identify proteins in the m. longissimus dorsi between early (12 months of age) and late (27 months of age) fattening stages of Hanwoo (Korean cattle) steers. Using two-dimensional electrophoresis and mass spectrometry, 8 proteins of 11 differentially expressed spots between the 12 and 27 month age groups were identified in the loin muscle. Among those that were differentially expressed, zinc finger 323 and myosin light chain were highly expressed in late-fattening stage, and two catabolic enzymes, triosephosphate isomerase (TPI) and succinate dehydrogenase (SDH) were expressed more in the early versus the late-fattening stage. In particular, the quantification of TPI and SDH by immunoblotting correlated well with fat content. Our data suggested that TPI and SDH are potential candidates as markers and their identification provides new insight into the molecular mechanisms and pathways associated with intramuscular fat contents of bovine skeletal muscle.
本研究的目的是鉴定韩牛（韩国肉牛）阉牛育肥早期（12月龄）和晚期（27月龄）背最长肌中的蛋白质。利用二维电泳和质谱分析，在腰大肌中鉴定出12月龄和27月龄组之间11个差异表达斑点中的8种蛋白质。在差异表达的蛋白质中，锌指323和肌球蛋白轻链在育肥后期高表达，而两种分解代谢酶，磷酸丙糖异构酶（TPI）和琥珀酸脱氢酶（SDH）在育肥早期比后期表达更多。特别是，通过免疫印迹对TPI和SDH进行定量分析，其结果与脂肪含量密切相关。我们的数据表明，TPI和SDH是作为标记物的潜在候选者，它们的鉴定为与牛骨骼肌肌内脂肪含量相关的分子机制和途径提供了新的见解。
