Lu E, Llano D A, Sherman S M
Department of Neurobiology, University of Chicago, Chicago, IL 60637, USA.
Hear Res. 2009 Nov;257(1-2):16-23. doi: 10.1016/j.heares.2009.07.009. Epub 2009 Jul 28.
We studied the distributions of calretinin and calbindin immunoreactivity in subdivisions of the mouse medial geniculate body and the adjacent paralaminar nuclei. We found that the vast majority of labeled cells in the dorsal division of the medial geniculate body were immunoreactive for calbindin-only, whereas most of the remaining labeled cells were double-labeled. Very few calretinin+ only cells were observed. By contrast, we observed significant proportions of calbindin+ only, calretinin+ only and double-labeled cells in the medial division of the medial geniculate body. Further, the distributions of calbindin-only, calretinin-only and double-labeled cells did not differ between the medial division of the medial geniculate body, the suprageniculate nucleus, the peripeduncular nucleus and the posterior intralaminar nucleus. We found essentially no somatic staining for either calbindin or calretinin in the ventral division of the medial geniculate body. These data suggest that there are distinct neurochemical differences between the two non-lemniscal auditory thalamic nuclei. In addition, these data extend previous observations that the medial division of the medial geniculate body shares many properties with the paralaminar group of nuclei.
我们研究了小鼠内侧膝状体各亚区及相邻的旁层核中钙结合蛋白和钙视网膜蛋白免疫反应性的分布。我们发现，内侧膝状体背侧部绝大多数标记细胞仅对钙结合蛋白呈免疫反应，而其余大多数标记细胞为双标记。仅观察到极少数仅钙视网膜蛋白阳性的细胞。相比之下，我们在内侧膝状体内侧部观察到相当比例的仅钙结合蛋白阳性、仅钙视网膜蛋白阳性和双标记细胞。此外，在内侧膝状体内侧部、上膝状体核、脚周核和后内侧核中，仅钙结合蛋白、仅钙视网膜蛋白和双标记细胞的分布没有差异。我们在内侧膝状体腹侧部基本上未发现钙结合蛋白或钙视网膜蛋白的体细胞染色。这些数据表明，两个非lemniscal听觉丘脑核之间存在明显的神经化学差异。此外，这些数据扩展了先前的观察结果，即内侧膝状体内侧部与旁层核群具有许多共同特性。
