Laboratorio de Veterquímica, Camino Melipilla 5641, Cerrillos, Santiago, Chile.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3001-5. doi: 10.1099/ijs.0.007021-0. Epub 2009 Jul 30.
Eight bacterial strains isolated from diseased rainbow trout (n=5) and Atlantic salmon (n=3) were characterized by phenotypic and molecular taxonomic methods. The isolates were negative for the Gram-reaction, non-motile, rod-shaped and catalase- and oxidase-positive. Colonies on solid media were yellow, smooth, shiny and circular with regular edges. Growth occurred at 4-28 degrees C (optimum, 15 degrees C) and with 0-3 % NaCl (optimum, 0.5 %). Analysis of the 16S rRNA gene sequence allocated the micro-organisms to the genus Chryseobacterium, with Chryseobacterium soldanellicola PSD1-4(T) and Chryseobacterium soli JS6-6(T) as their closest relatives (96.9 and 97.1 % sequence similarity, respectively). The levels of DNA-DNA hybridization towards these nearest phylogenetic neighbours were below 17.1 %. The DNA G+C contents of strains VQ-6316s(T) and VQ-4836s were 32.5 and 32.3 mol%, respectively. The predominant menaquinone was MK-6 and the major fatty acids were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(17 : 1)omega9c, iso-C(17 : 0) 3-OH and summed feature 3 (comprising C(16 : 1)omega7c and/or C(16 : 1)omega7t and/or iso-C(15 : 0) 2-OH). The eight isolates were classified as representatives of a novel species, Chryseobacterium piscicola sp. nov., with strain VQ-6316s(T) (=CECT 7357(T)=DSM 21068(T)) as the type strain.
从患病虹鳟鱼（n=5）和大西洋鲑鱼（n=3）中分离出的 8 株细菌通过表型和分子分类学方法进行了鉴定。这些分离物革兰氏反应阴性，不运动，呈杆状，过氧化氢酶和氧化酶阳性。固体培养基上的菌落呈黄色、光滑、有光泽、圆形且边缘规则。生长温度为 4-28°C（最适温度为 15°C），盐度为 0-3%（最适盐度为 0.5%）。16S rRNA 基因序列分析将微生物分配到黄杆菌属，其中 Chryseobacterium soldanellicola PSD1-4(T) 和 Chryseobacterium soli JS6-6(T) 是它们最接近的亲缘关系（序列相似度分别为 96.9%和 97.1%）。与这些最近的系统发育近邻的 DNA-DNA 杂交水平低于 17.1%。菌株 VQ-6316s(T) 和 VQ-4836s 的 DNA G+C 含量分别为 32.5%和 32.3mol%。主要的menaquinone 是 MK-6，主要脂肪酸是 iso-C(15 : 0)、anteiso-C(15 : 0)、iso-C(17 : 1)omega9c、iso-C(17 : 0)3-OH 和 sum feature 3（包括 C(16 : 1)omega7c 和/或 C(16 : 1)omega7t 和/或 iso-C(15 : 0)2-OH）。这 8 株分离物被分类为一种新型物种，即鱼类黄杆菌（Chryseobacterium piscicola）sp. nov.，其中 VQ-6316s(T) 菌株（=CECT 7357(T)=DSM 21068(T)）为模式菌株。
