Kämpfer Peter, Vaneechoutte Mario, Lodders Nicole, De Baere Thierry, Avesani Véronique, Janssens Michèle, Busse Hans-Jürgen, Wauters Georges
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2421-8. doi: 10.1099/ijs.0.008250-0. Epub 2009 Jul 21.
A collection of eight strains, NF 1366(T), NF 450, NF 1101, NF 1107, NF 1123, NF 1413, CCUG 15260 and CCUG 15624, from various clinical origins, were characterized biochemically as similar to Kaistella koreensis and Chryseobacterium haifense. They differed from K. koreensis, which is unable to alkalinize acetate, and from C. haifense, which is ONPG-positive (beta-galactosidase) and acidifies sucrose, fructose and lactose. Based on 16S rRNA gene sequence comparisons, this collection of strains was most closely related to the type strains of K. koreensis (97.3-97.5 %) and C. haifense (99.1 %). Representative strain NF 1366(T) showed only 41.8 % DNA-DNA relatedness with K. koreensis DSM 12107(T) and only 51.9 % with C. haifense DSM 19056(T). DNA-DNA hybridization of strains NF 450 and CCUG 15624 to strain NF 1366(T) was 41.7 and 74.6 %, respectively, and relatedness of these strains with C. haifense DSM 19056(T) was 72.6 and 70.2 %. With the present information, these two strains must be classified as intermediate between C. haifense and strain NF 1366(T). The fatty acid composition and polar lipid profile of strain NF 1366(T) were similar to those reported for other Chryseobacterium species. Like other chryseobacteria, strain NF 1366(T) exhibited a polyamine pattern with the predominant compound sym-homospermidine and a quinone system consisting of menaquinone MK-6 only. For this collection of clinical strains, the name Chryseobacterium anthropi sp. nov. is proposed, with NF 1366(T) (=CCUG 52764(T) =CIP 109762(T)) as the type strain. K. koreensis was shown to be very similar genotypically and phenotypically to Chryseobacterium. Its polar lipid profile exhibited the major characteristics shown for recently described Chryseobacterium species and the fatty acid profile of K. koreensis was also very similar to those of the Chryseobacterium species. Hence, no striking genotypic or phenotypic differences could be found that could justify the classification of this species into a separate genus, and we therefore propose to reclassify Kaistella koreensis in the genus Chryseobacterium as Chryseobacterium koreense comb. nov. (type strain Chj707(T) =IAM 15050(T) =JCM 21512(T) =KCTC 12107(T) =NBRC 103027(T)). An emended description of the genus Chryseobacterium is also proposed.
从不同临床来源收集的8株菌株，即NF 1366(T)、NF 450、NF 1101、NF 1107、NF 1123、NF 1413、CCUG 15260和CCUG 15624，经生化鉴定与韩国凯斯氏菌（Kaistella koreensis）和海夫金黄杆菌（Chryseobacterium haifense）相似。它们与不能使乙酸盐碱化的韩国凯斯氏菌以及ONPG阳性（β-半乳糖苷酶）且能使蔗糖、果糖和乳糖酸化的海夫金黄杆菌不同。基于16S rRNA基因序列比较，这组菌株与韩国凯斯氏菌的模式菌株（97.3 - 97.5%）和海夫金黄杆菌的模式菌株（99.1%）关系最为密切。代表性菌株NF 1366(T)与韩国凯斯氏菌DSM 12107(T)的DNA - DNA相关性仅为41.8%，与海夫金黄杆菌DSM 19056(T)的DNA - DNA相关性仅为51.9%。菌株NF 450和CCUG 15624与菌株NF 1366(T)的DNA - DNA杂交率分别为41.7%和74.6%，这些菌株与海夫金黄杆菌DSM 19056(T)的相关性分别为72.6%和70.2%。根据现有信息，这两株菌株必须归类为介于海夫金黄杆菌和菌株NF 1366(T)之间的中间类型。菌株NF 1366(T)的脂肪酸组成和极性脂质谱与其他金黄杆菌属物种报道的相似。与其他金黄杆菌一样，菌株NF 1366(T)呈现出以主要化合物对称高亚精胺为主的多胺模式和仅由甲基萘醌MK - 6组成的醌系统。对于这组临床菌株，提议命名为人类金黄杆菌（Chryseobacterium anthropi sp. nov.），以NF 1366(T)（=CCUG 52764(T) =CIP 109762(T)）作为模式菌株。结果表明，韩国凯斯氏菌在基因型和表型上与金黄杆菌非常相似。其极性脂质谱呈现出最近描述的金黄杆菌属物种的主要特征，韩国凯斯氏菌的脂肪酸谱也与金黄杆菌属物种的脂肪酸谱非常相似。因此，未发现明显的基因型或表型差异足以证明将该物种归入一个单独的属是合理的，所以我们提议将韩国凯斯氏菌重新分类到金黄杆菌属，命名为韩国金黄杆菌（Chryseobacterium koreense comb. nov.）（模式菌株Chj707(T) =IAM 15050(T) =JCM 21512(T) =KCTC 12107(T) =NBRC 103027(T)）。同时还提议对金黄杆菌属进行修订描述。
