保加利亚轮状病毒肠胃炎的五年研究。

Five year study of rotavirus gastroenteritis in Bulgaria.

作者信息

Shindarov L M, Dimitrov D H, Rangelova S, Popov G, Tcakov B, Tsilka E

机构信息

Medical Academy Research Institute of Infectious ans Parasitic Diseases, Department of Virology, Sofia, Bulgaria.

出版信息

Acta Virol. 1988 Jul;32(4):309-16.

PMID:2903635

Abstract

We tested by electron microscopy 7,530 samples from children admitted to different hospitals in Sofia between February 1981 and February 1986; from these, rotaviruses were found in 725 (9.6%) faecal samples. Electron microscopic analysis of 264 samples from 181 children admitted to the hospital of infectious diseases in Shumen between December 1984 to February 1986 revealed rotaviruses in 120 (66.6%) of the tested children. A part of the samples positive by electron microscopy was tested by ELISA and polyacrylamide gel electrophoresis for rotavirus RNA segment patterns. Rotaviruses with eight different electropherotypes were found in Bulgaria. The seasonal culmination of the rotavirus gastroenteritis in the winter months has been confirmed. Rotavirus antibodies were found in 73% of the sera from 152 children tested.

摘要

1981年2月至1986年2月期间，我们对索非亚不同医院收治的儿童的7530份样本进行了电子显微镜检测；其中，在725份（9.6%）粪便样本中发现了轮状病毒。对1984年12月至1986年2月期间在舒门传染病医院收治的181名儿童的264份样本进行电子显微镜分析，结果显示，在120名（66.6%）受试儿童中发现了轮状病毒。对部分经电子显微镜检测呈阳性的样本进行了ELISA和聚丙烯酰胺凝胶电泳，以检测轮状病毒RNA片段模式。在保加利亚发现了具有八种不同电泳型的轮状病毒。已证实轮状病毒性肠胃炎在冬季达到季节性高峰。在152名受试儿童的血清中，73%检测出轮状病毒抗体。