Luo Qi-Zhi, Dai Kai-Jin, Ma An-De
Southern Medical University, Guangzhou 510515, China.
Zhong Yao Cai. 2009 Apr;32(4):526-9.
To study and compare the main chemical constituents of Sarcandra glabra and qingrexiaoyanning capsules which were extracted by acetic ether.
The sample solution were analyzed by a Zorbax C18 column with a gradient mobile phase composed of acetonitrile and 0.1% formic acid solution. Both UV and mass spectrometry detector were used simutaneously, full-scan detection mode was evaluated for the identification of all LC peaks.
We analyzed the mass spectrum of every LC peak and identified 26 molecular mass from the ion chromatogram of Sarcandra glabra extraction and 16 molecular mass from the extractions of qingrexiaoyanning capsule. 5 compounds were identified.
High performance liquid chromatography-mass/mass spectrometry has special advantages on analyzing the chemical constituents of traditional Chinese medicine.
研究并比较肿节风和清热消炎宁胶囊经乙酸乙酯提取后的主要化学成分。
采用Zorbax C18色谱柱，以乙腈和0.1%甲酸溶液组成的梯度流动相进行分析。同时使用紫外和质谱检测器，采用全扫描检测模式对所有液相色谱峰进行鉴定。
分析了各液相色谱峰的质谱图，从肿节风提取物的离子色谱图中鉴定出26个分子量，从清热消炎宁胶囊提取物中鉴定出16个分子量。鉴定出5种化合物。
高效液相色谱-质谱/质谱联用技术在分析中药化学成分方面具有独特优势。
