Liu Jian-Xin, Liu Yong, Chen Xin-Lin, Zhao Jian-Jun, Song Tu-Sheng, Qian Yi-Hua
Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Institute of Neurobiology, School of Medicine, Xi'an Jiaotong University, Xi'an 710006, China.
Zhong Yao Cai. 2009 Apr;32(4):548-56.
To investigate the effects of breviscapine on the functions of spatial learning and memory of focal cerebral ischemia rats.
Rots withl the left middle cerebral artery occluded were made by an intraluminal filament. Then breviscapine (20 mg/kg,40 mg/kg) in experimental group and 10% glucose in control group were administered intraperitoneally once a day for 2 weeks, and the Morris water maze tasks were carried out for 5 days.
Compared with sham-operation group,the animals of ischemia-control group exhibited seriously impaired spatial learning and memory in both place navigation test and spatial probe test. In the place navigation test, the mean value of escape latency in breviscapine group was significantly shorter than that in ischemia control group (P < 0.01 for lower-dose and P < 0.05 for higher-dose breviscapine group, respectively). In the spatial probe test,compared with sham-operation (P < 0.01) and breviscapine group (P < 0.01), the rats of ischemia-control group spent more time in the no-former platform quadrant, and showed reduced frequency of crossing former platform site significantly. The numbers of neurons with Nissl staining and choline acetyltransferase (ChAT) immunopositive neurons in ipssilateral cortex in the breviscapine group were significantly more than those in the ischemia-control group (P < 0.01). In hippocampus, the numbers of neurons with Nissl staining and ChAT immunopositive neurons in the stratum pyramidale of CA area were similar among the groups.
These results indicate that breviscapine can improve the functions of spatial learning and memory of focal cerebral ischemia rats and the protection against the loss of ChAT immunopositive neuron in new cortex may be involved in its mechanisms.
探讨灯盏花素对局灶性脑缺血大鼠空间学习记忆功能的影响。
采用线栓法制备左侧大脑中动脉闭塞大鼠模型。将实验大鼠分为灯盏花素低剂量组（20mg/kg）、高剂量组（40mg/kg），对照组腹腔注射10%葡萄糖，均每日1次，连续给药2周，并进行5天的Morris水迷宫实验。
与假手术组比较，缺血对照组大鼠在定位航行实验和空间探索实验中的空间学习记忆能力明显受损。在定位航行实验中，灯盏花素低、高剂量组大鼠逃避潜伏期均值均显著短于缺血对照组（低剂量组P<0.01，高剂量组P<0.05）。在空间探索实验中，缺血对照组大鼠在原平台象限停留时间明显延长，穿越原平台次数明显减少，与假手术组（P<0.01）和灯盏花素组（P<0.01）比较差异有统计学意义。灯盏花素组大鼠大脑皮质尼氏染色阳性神经元数及胆碱乙酰转移酶（ChAT）免疫阳性神经元数均明显多于缺血对照组（P<0.01）。海马CA区锥体细胞层尼氏染色阳性神经元数及ChAT免疫阳性神经元数在各组间差异无统计学意义。
灯盏花素可改善局灶性脑缺血大鼠的空间学习记忆功能，其机制可能与保护大脑皮质ChAT免疫阳性神经元有关。
