Tian Jin-Zhou, Yin Jun-Xiang, Cheng Long, Shi Jing, Li Xiao-Li, Xu Yi, Song Cong-Sun, Ren Ying, Huang Qi-Fu, Wang Yong-Yan
Institutes of Geriatrics, Beijing University of Chinese Medicine, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(6):480-2, 521.
To explore the influence of Kingsbrain (GETO) on the learning memory impairment of rats with cerebral ischemia.
Rats with cerebral ischemia were administered GETO orally once a day for one month. The ability of spatial-learning memory of rats was evaluated by Morris Water Maze (MWM). Duxil was used as a positive control.
the results of place navigation of MWM showed that at the 3rd time of swimming training, the escape latency of rats of the GETO group, Duxil group and Sham group were shorter than that of model group. The escape latency were (54.1 +/- 43.94), (55.9 +/- 43.49), (50.4 +/- 34.99) and (85.4 +/- 42.8) s, respectively; but there was no significantly difference. After the 6th time of swimming training, the escape latency of rats of the GETO group (37.8 +/- 38.69) s, the Duxil group (37.4 +/- 38.03) s and the sham group (26.9 +/- 21.63) s were significantly shorter than that of model rats (77.5 +/- 47.59) s, P < 0.05, respectively. Comparison of the swimming distance among groups were similar to the escape latency among groups. In the test of spatial probe, results of the ratio of the swimming time of platform quadrant (tP) vs the total swimming time (tT) and the ratio of the swimming distance of platform quadrant (dP) vs the total swimming distance (dT) indicated that the ratios of the GETO group (0.347 +/- 0.0662, 0.344 +/-0.055 1), the Duxil group (0.345 +/- 0.0984, 0.34 +/- 0.0934) and the sham group (0.35 +/- 0.0662, 0.349 +/- 0.0589) were significantly higher than those of the Model group (0.261 +/- 0.0689, 0.274 +/- 0.0544), P < 0.05, respectively.
GETO can significantly improve the spatial learning and memory ability of rats with cerebral ischemia, which provides the pharmacodynamics evidence for its clinical application of improveing the learning and memory ability in poststroke patients.
探讨脑力智宝（GETO）对脑缺血大鼠学习记忆障碍的影响。
对脑缺血大鼠每日灌胃给予GETO 1次，连续1个月。采用Morris水迷宫（MWM）评价大鼠的空间学习记忆能力。以都可喜作为阳性对照。
MWM定位航行实验结果显示，在游泳训练第3次时，GETO组、都可喜组和假手术组大鼠的逃避潜伏期均短于模型组，逃避潜伏期分别为（54.1±43.94）、（55.9±43.49）、（50.4±34.99）和（85.4±42.8）秒，但差异无统计学意义。游泳训练第6次后，GETO组（37.8±38.69）秒、都可喜组（37.4±38.03）秒和假手术组（26.9±21.63）秒大鼠的逃避潜伏期均显著短于模型大鼠（77.5±47.59）秒，P<0.05。各组间游泳距离比较结果与逃避潜伏期比较结果相似。在空间探索实验中，平台象限游泳时间（tP）与总游泳时间（tT）的比值以及平台象限游泳距离（dP）与总游泳距离（dT）的比值结果显示，GETO组（0.347±0.0662，0.344±0.0551）、都可喜组（0.345±0.0984，0.34±0.0934）和假手术组（0.35±0.0662，0.349±0.0589）的比值均显著高于模型组（0.261±0.0689，0.274±0.0544），P<0.05。
GETO可显著提高脑缺血大鼠的空间学习记忆能力，为其改善脑卒中后患者学习记忆能力的临床应用提供了药效学依据。
