白三烯B4应用后多形核白细胞经皮迁移的超微结构研究

An ultrastructural study of transcutaneous migration of polymorphonuclear leukocytes following application of leukotriene B4.

作者信息

Paulissen M, Peereboom-Stegeman J H, van de Kerkhof P C

机构信息

Department of Toxicology, University of Nijmegen, The Netherlands.

出版信息

Skin Pharmacol. 1990;3(4):236-47. doi: 10.1159/000210876.

DOI:10.1159/000210876

PMID:1964566

Abstract

In the present study the ultrastructural aspects of the migration of polymorphonuclear leukocytes (PMN) in apparently normal skin following the epicutaneous application of leukotriene B4 (LTB4) were studied and it was investigated whether this model is representative of the migration of PMN in psoriatic skin. At the ultrastructural level the following features were observed: degranulation of PMN, formation of fenestrations and gaps of the endothelium, a multilayered basal lamina and hyperactive endothelial cells with protrusions and focal necrosis of these cells. Epicutaneous application of LTB4 is a practical approach to study the dynamics of the interaction between PMN and the endothelium in vivo.

摘要

在本研究中，我们研究了在表皮应用白三烯B4（LTB4）后，多形核白细胞（PMN）在看似正常的皮肤中迁移的超微结构特征，并研究了该模型是否代表PMN在银屑病皮肤中的迁移。在超微结构水平上观察到以下特征：PMN脱颗粒、内皮细胞窗孔和间隙形成、多层基膜以及内皮细胞活跃，伴有突起和这些细胞的局灶性坏死。表皮应用LTB4是一种在体内研究PMN与内皮细胞相互作用动态的实用方法。