Pesce Stéphane, Batisson Isabelle, Bardot Corinne, Fajon Céline, Portelli Christophe, Montuelle Bernard, Bohatier Jacques
CEMAGREF, UR MALY, 3bis Quai Chauveau CP 220 69336 Lyon, France.
Ecotoxicol Environ Saf. 2009 Oct;72(7):1905-12. doi: 10.1016/j.ecoenv.2009.07.004. Epub 2009 Jul 30.
Seasonal variation in the response of riverine microbial communities to an environmentally relevant exposure to glyphosate (about 10 microgl(-1)) was assessed on natural communities collected in spring and summer, using two 14-day microcosm studies. The two experiments showed no major effect of glyphosate on algal biomass (chlorophyll a concentrations), bacterial activity ([(3)H]thymidine incorporation), or bacterial community diversity (16S PCR-TTGE detection). Effects on algal community composition (genus-level taxonomic identification) and eukaryotic community diversity (18S PCR-DGGE on <100 microm organisms) were only detected on the samples collected in summer. This work demonstrates that even if the effects of a short pulse of glyphosate (10 microgl(-1)) on riverine microorganisms seem to be limited, the responses of natural microbial communities to glyphosate exposure (and probably to other pesticide exposures) can clearly vary between the experiments, and can be seasonally dependent.
利用两项为期14天的微观世界研究，对春季和夏季采集的自然群落进行评估，以研究河流微生物群落对与环境相关的草甘膦暴露（约10微克/升）的反应中的季节性变化。这两项实验表明，草甘膦对藻类生物量（叶绿素a浓度）、细菌活性（[³H]胸腺嘧啶核苷掺入）或细菌群落多样性（16S PCR-TTGE检测）没有重大影响。仅在夏季采集的样本中检测到对藻类群落组成（属级分类鉴定）和真核生物群落多样性（对<100微米的生物体进行18S PCR-DGGE）的影响。这项工作表明，即使草甘膦短脉冲（10微克/升）对河流微生物的影响似乎有限，但自然微生物群落对草甘膦暴露（可能还有其他农药暴露）的反应在不同实验之间可能明显不同，并且可能具有季节性依赖性。
