de Mattos Carvalho Armando, Alves Ana Liz Garcia, Golim Marjorie Assis, Moroz Andrei, Hussni Carlos Alberto, de Oliveira Patrícia Galvão Gomes, Deffune Elenice
Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil.
Vet Immunol Immunopathol. 2009 Dec 15;132(2-4):303-6. doi: 10.1016/j.vetimm.2009.06.014. Epub 2009 Jun 30.
The purpose of this work was to isolate and cultivate mesenchymal stem cells (MSC) derived from equine adipose tissue and conduct cellular characterization with the following markers: CD90, CD44 and CD13. Adipose tissue collection was performed at the base of the horses' tails, followed by immediate isolation and cultivation of the MSC and posterior characterization by flow cytometry for the interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), fluorescein isothiocyanate (FITC), and tests with specific mAb mouse anti-horse CD13 and mouse anti-horse CD44. The technique used for isolation and cell cultivation proved to be safe and viable. The CD90 mAb expressed cross-reaction with MSC derived from equine adipose tissue and CD44 showed greater expression in cells as the number of culture passages increased. Although marker CD13 expresses reaction in other studies involving MSC in different species, it presented no expression in the experiment realized. The results obtained revealed the immunophenotypic characterization of the surface of isolated and cultivated MSC, classifying these cells as a promising type of progenitor cells that can be applied in equine cellular therapy.
本研究的目的是分离和培养源自马脂肪组织的间充质干细胞（MSC），并用以下标志物进行细胞表征：CD90、CD44和CD13。在马尾巴根部采集脂肪组织，随后立即分离和培养MSC，并通过流式细胞术进行后续表征，使用小鼠抗大鼠CD90单克隆抗体（mAb）进行种间反应试验、异硫氰酸荧光素（FITC）以及用小鼠抗马CD13和小鼠抗马CD44特异性mAb进行检测。用于分离和细胞培养的技术被证明是安全可行的。CD90 mAb与源自马脂肪组织的MSC表现出交叉反应，并且随着培养代数的增加，CD44在细胞中的表达更高。尽管在其他涉及不同物种MSC的研究中标志物CD13表现出反应，但在本实验中未呈现表达。所获得的结果揭示了分离和培养的MSC表面的免疫表型特征，将这些细胞归类为一种有前景的祖细胞类型，可应用于马的细胞治疗。
