Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
Departamento de Microbiologia, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):244-248. doi: 10.1099/ijs.0.011577-0. Epub 2009 Jul 31.
Two novel yeast species, Candida aechmeae sp. nov. and Candida vrieseae sp. nov., were isolated from bromeliads in Itapuã Park, Rio Grande do Sul, Brazil. These species are genetically isolated from all other currently recognized ascomycetous yeasts based on their sequence divergence in the D1/D2 domain of the LSU rRNA gene. C. aechmeae sp. nov. is phylogenetically close to Candida ubatubensis, a species also isolated from bromeliads in Brazil, but the novel species can be differentiated on the basis of differences in the D1/D2 domain and positive results for the assimilation of l-arabinose, raffinose, inulin and citrate. Candida vrieseae sp. nov. is phylogenetically placed in a clade near Candida membranifaciens that is composed of several species associated with insects, but the novel species can be differentiated from them by the D1/D2 and ITS gene sequences, positive results for the assimilation of nitrite and a negative result for the assimilation of ethylamine. The type strain for Candida aechmeae sp. nov. is BI153(T) (=CBS 10831(T)=NRRL Y-48456(T)) and the type strain for C. vrieseae sp. nov. is BI146(T) (=CBS 10829(T)=NRRL Y-48461(T)).
两个新的酵母物种，即 Candida aechmeae sp. nov. 和 Candida vrieseae sp. nov.，从巴西伊塔普阿公园的凤梨科植物中分离出来。这些物种基于其 LSU rRNA 基因 D1/D2 区的序列差异，与所有其他已识别的子囊菌酵母在遗传上是隔离的。C. aechmeae sp. nov. 在系统发育上与 Candida ubatubensis 密切相关，后者也是从巴西的凤梨科植物中分离出来的，但新物种可以通过 D1/D2 区的差异以及对 l-阿拉伯糖、棉子糖、菊粉和柠檬酸盐同化的阳性结果来区分。Candida vrieseae sp. nov. 在系统发育上位于一个与昆虫相关的多个物种组成的分支附近，靠近 Candida membranifaciens，但新物种可以通过 D1/D2 和 ITS 基因序列、对亚硝酸盐同化的阳性结果以及对乙胺同化的阴性结果来与它们区分开来。Candida aechmeae sp. nov. 的模式株为 BI153(T) (=CBS 10831(T)=NRRL Y-48456(T))，Candida vrieseae sp. nov. 的模式株为 BI146(T) (=CBS 10829(T)=NRRL Y-48461(T))。
