Landell Melissa Fontes, Brandão Luciana R, Safar Silvana V B, Gomes Fatima C O, Félix Ciro R, Santos Ana Raquel O, Pagani Danielle M, Ramos Jesus P, Broetto Leonardo, Mott Tamí, Vainstein Marilene H, Valente Patricia, Rosa Carlos A
Programa de Pós-Graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Setor de Genética/ICBS, Universidade Federal de Alagoas, Maceió - AL, Brazil.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2466-2471. doi: 10.1099/ijs.0.000285. Epub 2015 Apr 24.
Two independent surveys of yeasts associated with different bromeliads in different Brazilian regions led to the proposal of a novel yeast species, Bullera vrieseae sp. nov., belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences in the internal transcribed spacer (ITS) region and D1/D2 domain of the LSU rRNA gene suggested affinity to a phylogenetic lineage that includes Bullera miyagiana and Bullera sakaeratica. Six isolates of the novel species were obtained from different bromeliads and regions in Brazil. Sequence analysis of the D1/D2 domains of the large subunit of the rRNA gene showed that the novel species differs from B. miyagiana and B. sakaeratica by 85 and 64 nt substitutions, respectively and by more than 75 nt substitutions in the ITS region. Phenotypically, Bullera vrieseae sp. nov. can be distinguished from both species based on the assimilation of meso-erythritol, which was negative for B. vrieseae sp. nov. but positive for the others, assimilation of d-glucosamine, which was positive for B. vrieseae sp. nov. but negative for B. miyagiana and of l-sorbose, which was negative for B. vrieseae sp. nov. but positive for B. sakaeratica. The novel species Bullera vrieseae sp. nov. is proposed to accommodate these isolates. The type strain of Bullera vrieseae sp. nov. is UFMG-CM-Y379T (BRO443T; ex-type CBS 13870T).
在巴西不同地区，对与不同凤梨科植物相关的酵母进行了两项独立调查，由此提出了一个新的酵母物种——弗里塞氏布勒酵母（Bullera vrieseae sp. nov.），它属于银耳目分支（伞菌亚门，担子菌门）。对内部转录间隔区（ITS）序列和 LSU rRNA 基因的 D1/D2 结构域进行分析表明，该物种与一个系统发育谱系具有亲缘关系，该谱系包括宫城布勒酵母（Bullera miyagiana）和坂仓布勒酵母（Bullera sakaeratica）。从巴西不同的凤梨科植物和地区获得了该新物种的六个分离株。对 rRNA 基因大亚基的 D1/D2 结构域进行序列分析表明，该新物种与宫城布勒酵母和坂仓布勒酵母在 D1/D2 结构域分别有 85 个和 64 个核苷酸替换，在 ITS 区域有超过 75 个核苷酸替换。在表型上，弗里塞氏布勒酵母新物种可以根据中赤藓糖醇的同化作用与这两个物种区分开来，中赤藓糖醇对弗里塞氏布勒酵母新物种的同化作用为阴性，但对其他物种为阳性；d - 葡萄糖胺的同化作用对弗里塞氏布勒酵母新物种为阳性，但对宫城布勒酵母为阴性；l - 山梨糖的同化作用对弗里塞氏布勒酵母新物种为阴性，但对坂仓布勒酵母为阳性。提出将这些分离株归为新物种弗里塞氏布勒酵母（Bullera vrieseae sp. nov.）。弗里塞氏布勒酵母新物种的模式菌株是 UFMG - CM - Y379T（BRO443T；模式菌株 CBS 13870T）。
