Felix Ciro R, Navarro Hector Mauricio Casanova, Paulino Gustavo Vasconcelos Bastos, Broetto Leonardo, Landell Melissa Fontes
Programa de Pós-graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Campus A. C. Simões, Av. Lourival Melo Mota, s/n, CEP 57072-900, Maceió, AL, Brazil.
Universidade Federal de Alagoas, Maceió, AL, Brazil.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1752-1757. doi: 10.1099/ijsem.0.001856. Epub 2017 Jun 14.
Yeast surveys associated with different bromeliads in north-eastern Brazil led to the proposal of two novel yeast species, Carlosrosaea hohenbergiae sp. nov. and Carlosrosaea aechmeae sp. nov., belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences of the internal transcribed spacer (ITS) region and D1/D2 domain of the LSU rRNA gene suggested an affinity with a phylogenetic lineage that includes recently reclassified Carlosrosaeavrieseae. Six isolates of the novel species were obtained from different bromeliad species collected in three Atlantic Forest fragments in Alagoas state, Brazil. Ca. hohenbergiae sp. nov. differs by 69 and 12 nucleotide substitutions in the ITS and D1/D2 domain, respectively, from Ca. vrieseae. The type strain is UFMG-CM-Y405T (=BSB 34T=CBS 14563T), Mycobank 819227. Ca. aechmeae sp. nov. is represented by one strain isolated from Aechmea constantinii leaves. Ca. aechmeae sp. nov. differs from the related species Ca. hohenbergiae and Ca. vrieseae by 36 and 65 nucleotide substitutions, respectively, in the ITS region and by 12 and 15 nucleotide substitutions in the D1/D2 domain, respectively. The type strain of Ca. aechmeae sp. nov. is UFMG-CM-Y6095T (=BM 94T=CBS 14578), Mycobank 819228.
对巴西东北部不同凤梨科植物相关酵母的调查，促成了两个新酵母物种的提议，即霍氏卡洛斯罗莎酵母（Carlosrosaea hohenbergiae sp. nov.）和康氏卡洛斯罗莎酵母（Carlosrosaea aechmeae sp. nov.），它们属于银耳目分支（伞菌亚门，担子菌门）。对内部转录间隔区（ITS）序列以及 LSU rRNA 基因的 D1/D2 结构域的分析表明，其与一个系统发育谱系具有亲缘关系，该谱系包括最近重新分类的弗里塞卡洛斯罗莎酵母（Carlosrosaeavrieseae）。从巴西阿拉戈斯州三个大西洋森林片段采集的不同凤梨科植物物种中获得了六个新物种的分离株。霍氏卡洛斯罗莎酵母新物种在 ITS 和 D1/D2 结构域中分别与弗里塞卡洛斯罗莎酵母有 69 个和 12 个核苷酸替换差异。模式菌株为 UFMG-CM-Y405T（=BSB 34T=CBS 14563T），保藏编号为 Mycobank 819227。康氏卡洛斯罗莎酵母新物种由从康氏光萼荷（Aechmea constantinii）叶片分离出的一个菌株代表。康氏卡洛斯罗莎酵母新物种在 ITS 区域分别与相关物种霍氏卡洛斯罗莎酵母和弗里塞卡洛斯罗莎酵母有 36 个和 65 个核苷酸替换差异，在 D1/D2 结构域分别有 12 个和 15 个核苷酸替换差异。康氏卡洛斯罗莎酵母新物种的模式菌株为 UFMG-CM-Y6095T（=BM 94T=CBS 14578），保藏编号为 Mycobank 819228。
