Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):78-83. doi: 10.1099/ijs.0.008573-0. Epub 2009 Jul 31.
An aerobic, Gram-stain-negative, rod-shaped bacterial isolate, strain NH36A(T), was isolated from a sandy sediment sample from the South China Sea. Colonies of the isolate were dark orange on M2 agar. Optimal growth was observed at pH 7.0-8.5, 30 degrees C and in the presence of 0.5-4.0 % (w/v) NaCl. The major fatty acids were C(15 : 0), iso-C(15 : 0), anteiso-C(15 : 0), iso-C(15 : 1), iso-C(15 : 0) 3-OH, iso-C(17 : 0) 3-OH and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c). The DNA G+C content was 38.9 mol%. 16S rRNA gene sequence analysis revealed that strain NH36A(T) was most closely related to members of the genus Arenibacter, exhibiting 94.3-96.2 % sequence similarity to the type strains of Arenibacter species. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus Arenibacter. The name Arenibacter nanhaiticus sp. nov. is proposed and the type strain is NH36A(T) (=LMG 24842(T)=CCTCC AB 208315(T)=MCCC 1A04137(T)).
一株好氧、革兰氏阴性、杆状的细菌分离株，菌株 NH36A(T)，是从南海的沙质沉积物样本中分离出来的。该分离株的菌落呈暗橙色，在 M2 琼脂上。最佳生长条件为 pH7.0-8.5、30°C 和 0.5-4.0%（w/v）NaCl。主要脂肪酸为 C(15:0)、iso-C(15:0)、anteiso-C(15:0)、iso-C(15:1)、iso-C(15:0)3-OH、iso-C(17:0)3-OH 和 summed feature 3（包括 iso-C(15:0)2-OH 和/或 C(16:1)omega7c）。DNA G+C 含量为 38.9mol%。16S rRNA 基因序列分析表明，菌株 NH36A(T)与 Arenibacter 属的成员最为密切相关，与 Arenibacter 属的模式种的序列相似性为 94.3-96.2%。基于表型、化学分类和系统发育数据，该生物应被归类为 Arenibacter 属的一个新种的代表。建议采用 Arenibacter nanhaiticus sp. nov. 这一名称，其模式菌株为 NH36A(T)=LMG 24842(T)=CCTCC AB 208315(T)=MCCC 1A04137(T)。
