Clinical Medicine, Clinical College of Anhui Medical University, No. 15, Feicuilu, 230601, Hefei, Anhui, China.
Cytotechnology. 2009 Apr;59(3):191-200. doi: 10.1007/s10616-009-9211-2. Epub 2009 Aug 2.
The purpose of this study is to investigate in vitro and ex vivo effects of matrine on the growth of human lung cancer and hepatoma cells and the cancer cell migration as well as the expressions of related proteins in the cancer cells. Matrine significantly inhibited the in vitro and ex vivo growth of human non-small cell lung cancer A549 and hepatoma SMMC-7721 cells. Matrine induced the apoptosis in A549 and SMMC-7721 cells. Western blot analysis indicated that matrine dose-dependently down-regulated the expression of anti-apoptotic protein Bcl-2 and up-regulated the level of pro-apoptotic protein bax, eventually leading the reduction of ratios of Bcl-2/Bax proteins in A549 and SMMC-7721 cells. Furthermore, matrine significantly suppressed the A549 cell migration without reducing the cell viability. In addition, matrine dramatically reduced the secretion of vascular endothelial growth factor A in A549 cells. More importantly, matrine markedly enhanced the anticancer activity of anticancer agent trichostatin A (the histone deacetylase inhibitor) by strongly reducing the viability and/or the ratio of Bcl-2/Bax protein in A549 cells. Our findings suggest that matrine may have the broad therapeutic and/or adjuvant therapeutic application in the treatment of human non-small cell lung cancer and hepatoma.
本研究旨在探讨苦参碱对人肺癌和肝癌细胞体外和体外生长、癌细胞迁移以及癌细胞相关蛋白表达的影响。苦参碱显著抑制人非小细胞肺癌 A549 和肝癌 SMMC-7721 细胞的体外和体外生长。苦参碱诱导 A549 和 SMMC-7721 细胞凋亡。Western blot 分析表明，苦参碱呈剂量依赖性地下调抗凋亡蛋白 Bcl-2 的表达，并上调促凋亡蛋白 bax 的水平，最终导致 A549 和 SMMC-7721 细胞中 Bcl-2/Bax 蛋白比例降低。此外，苦参碱显著抑制 A549 细胞迁移而不降低细胞活力。此外，苦参碱还显著降低了 A549 细胞中血管内皮生长因子 A 的分泌。更重要的是，苦参碱通过强烈降低 A549 细胞的活力和/或 Bcl-2/Bax 蛋白的比例，显著增强了抗癌药物曲古抑菌素 A（组蛋白去乙酰化酶抑制剂）的抗癌活性。我们的研究结果表明，苦参碱可能在治疗人非小细胞肺癌和肝癌方面具有广泛的治疗和/或辅助治疗应用。
