茶氨酸及其在茶氨酸喂养大鼠血清对跨间皮细胞单层的受体介导的癌细胞侵袭的抑制作用。
Inhibitory effects of theanine and sera from theanine-fed rats on receptor-mediated cancer cell invasion beneath mesothelial-cell monolayers.
机构信息
Department of Applied Biological Science, Tokyo Noko University, Saiwaicho 3-5-8, Fuchu, Tokyo, 183-8509, Japan.
出版信息
Cytotechnology. 2001 Jul;36(1-3):195-200. doi: 10.1023/A:1014005423181.
Abstract
To investigate the bioavailability and mode of action of theanine against cancer, we examined in vitro and ex vivo effects of theanine on invasion of a rat ascites hepatoma cell line of AH109A. Theanine dose-dependently inhibited the invasion of AH109A cells across rat mesentery-derived mesothelial-cell (M-cell) monolayers without restraining AH109A cell proliferation in vitro. Rat sera obtained after oral intubation of theanine also inhibited the invasion. A competitive N-methyl-D-aspartate (NMDA) type glutamate receptor antagonist, (+/-) 2-amino-5-phosphonopentanoic acid (AP-5), dose-dependently counteracted the theanine-mediated in vitro and ex vivo inhibition of AH109A invasion. A competitive non-NMDA type glutamate receptor antagonist, 6,7-dinitroquinoxaline 2,3-dione (DNQX), did not affect this inhibition by theanine in vitro. These results suggest that the inhibition of AH109A invasion by theanine may be mediated by the NMDA receptor of AH109A.
摘要
为了研究茶氨酸对癌症的生物利用度和作用模式，我们研究了茶氨酸对 AH109A 大鼠腹水肝癌细胞系侵袭的体外和离体效应。茶氨酸剂量依赖性地抑制 AH109A 细胞穿过大鼠肠系膜来源的间皮细胞 (M 细胞) 单层的侵袭，而在体外不抑制 AH109A 细胞增殖。经口灌胃茶氨酸后获得的大鼠血清也抑制了侵袭。竞争性 N-甲基-D-天冬氨酸 (NMDA) 型谷氨酸受体拮抗剂 (+/-) 2-氨基-5-磷戊酸 (AP-5) 剂量依赖性地拮抗了茶氨酸介导的 AH109A 体外和离体侵袭抑制作用。竞争性非 NMDA 型谷氨酸受体拮抗剂 6,7-二硝基喹喔啉-2,3-二酮 (DNQX) 对茶氨酸在体外的这种抑制作用没有影响。这些结果表明，茶氨酸对 AH109A 侵袭的抑制可能是由 AH109A 的 NMDA 受体介导的。
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本文引用的文献
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Assay systems for screening food components that have anti-proliferative and anti-invasive activity to rat ascites hepatoma cells: In vitro and ex vivo effects of green tea extract.筛选具有抗增殖和抗侵袭活性的食物成分的分析系统：绿茶提取物的体外和体内效应。
Cytotechnology. 1997 Jan;23(1-3):127-32. doi: 10.1023/A:1007951231617.
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Inhibitory effect of curcumin on the invasion of rat ascites hepatoma cells in vitro and ex vivo.姜黄素对体外和体内大鼠腹水肝癌细胞侵袭的抑制作用。
Cytotechnology. 2001 Jan;35(1):57-63. doi: 10.1023/A:1008167502110.
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