Department of Applied Biological Science, Tokyo Noko University, Saiwaicho 3-5-8, Fuchu, Tokyo, 183-8509, Japan.
Cytotechnology. 2001 Jul;36(1-3):195-200. doi: 10.1023/A:1014005423181.
To investigate the bioavailability and mode of action of theanine against cancer, we examined in vitro and ex vivo effects of theanine on invasion of a rat ascites hepatoma cell line of AH109A. Theanine dose-dependently inhibited the invasion of AH109A cells across rat mesentery-derived mesothelial-cell (M-cell) monolayers without restraining AH109A cell proliferation in vitro. Rat sera obtained after oral intubation of theanine also inhibited the invasion. A competitive N-methyl-D-aspartate (NMDA) type glutamate receptor antagonist, (+/-) 2-amino-5-phosphonopentanoic acid (AP-5), dose-dependently counteracted the theanine-mediated in vitro and ex vivo inhibition of AH109A invasion. A competitive non-NMDA type glutamate receptor antagonist, 6,7-dinitroquinoxaline 2,3-dione (DNQX), did not affect this inhibition by theanine in vitro. These results suggest that the inhibition of AH109A invasion by theanine may be mediated by the NMDA receptor of AH109A.
为了研究茶氨酸对癌症的生物利用度和作用模式，我们研究了茶氨酸对 AH109A 大鼠腹水肝癌细胞系侵袭的体外和离体效应。茶氨酸剂量依赖性地抑制 AH109A 细胞穿过大鼠肠系膜来源的间皮细胞 (M 细胞) 单层的侵袭，而在体外不抑制 AH109A 细胞增殖。经口灌胃茶氨酸后获得的大鼠血清也抑制了侵袭。竞争性 N-甲基-D-天冬氨酸 (NMDA) 型谷氨酸受体拮抗剂 (+/-) 2-氨基-5-磷戊酸 (AP-5) 剂量依赖性地拮抗了茶氨酸介导的 AH109A 体外和离体侵袭抑制作用。竞争性非 NMDA 型谷氨酸受体拮抗剂 6,7-二硝基喹喔啉-2,3-二酮 (DNQX) 对茶氨酸在体外的这种抑制作用没有影响。这些结果表明，茶氨酸对 AH109A 侵袭的抑制可能是由 AH109A 的 NMDA 受体介导的。
