Yuan C F, Kao S M, Wang D C, Ng H T, Pao C C
Department of Obstetrics and Gynecology, Veteran General Hospital, Taipei, Taiwan, Republic of China.
Mol Cell Probes. 1990 Dec;4(6):475-83. doi: 10.1016/0890-8508(90)90006-l.
The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The sensitivities of detecting HCMV by in situ hybridization and PCR methods were 76.2% and 90.5% and the specificities were 92.1% and 90.5%, respectively, when compared with conventional culture method. The PCR compared favourably with both conventional culture and in situ hybridization methods and it may become a valuable and useful tool for the early and rapid detection of HCMV in clinical specimens.
对从相同数量孕妇处获取的388份宫颈阴道细胞标本进行了人巨细胞病毒（HCMV）检测。分别采用传统培养法、原位DNA杂交法和聚合酶链反应（PCR）法，在这些标本中检测到HCMV的比例分别为5.41%、11.6%和13.9%。与传统培养法相比，原位杂交法和PCR法检测HCMV的灵敏度分别为76.2%和90.5%，特异性分别为92.1%和90.5%。PCR法与传统培养法和原位杂交法相比具有优势，它可能成为临床标本中HCMV早期快速检测的一种有价值且有用的工具。
