Brytting M, Xu W, Wahren B, Sundqvist V A
Department of Virology, Karolinska Institute, Stockholm, Sweden.
J Clin Microbiol. 1992 Aug;30(8):1937-41. doi: 10.1128/jcm.30.8.1937-1941.1992.
Using a specific and sensitive polymerase chain reaction method, we detected reliably the presence of human cytomegalovirus (HCMV) DNA directly in serum samples collected at an early stage of HCMV infection, even before immunoglobulin M (IgM) antibodies were measurable. HCMV DNA was detected in serum from all patients with active HCMV infection; in 91% of these patients, HCMV DNA was found in the acute-phase serum. In 13 of 44 patients, HCMV DNA was found in serum before HCMV-specific IgM. For four kidney transplant recipients, the occurrence of HCMV DNA in serum, virus isolation from urine and leukocytes, and HCMV IgG and IgM serology were determined. We found a correlation between HCMV DNA in serum and positive virus isolation from leukocytes. In three of five congenitally infected infants, HCMV DNA and HCMV IgM were detected in the same sample. Two other infants were HCMV DNA positive, although no HCMV IgM antibodies were measurable. HCMV was found in urine from these infants either by virus isolation or with the polymerase chain reaction. Serum from one of the 22 healthy HCMV-seropositive blood donors was HCMV polymerase chain reaction positive.
我们采用一种特异且灵敏的聚合酶链反应方法，直接在人巨细胞病毒（HCMV）感染早期采集的血清样本中可靠地检测到了HCMV DNA，甚至在免疫球蛋白M（IgM）抗体可检测到之前。在所有活动性HCMV感染患者的血清中均检测到了HCMV DNA；其中91%的患者在急性期血清中发现了HCMV DNA。在44例患者中的13例中，在HCMV特异性IgM出现之前血清中就发现了HCMV DNA。对4例肾移植受者，测定了血清中HCMV DNA的出现情况、从尿液和白细胞中分离病毒的情况以及HCMV IgG和IgM血清学情况。我们发现血清中的HCMV DNA与从白细胞中分离出阳性病毒之间存在相关性。在5例先天性感染婴儿中的3例中，在同一样本中检测到了HCMV DNA和HCMV IgM。另外2例婴儿HCMV DNA呈阳性，尽管未检测到HCMV IgM抗体。通过病毒分离或聚合酶链反应在这些婴儿的尿液中发现了HCMV。在22例健康的HCMV血清阳性献血者中，有1例的血清HCMV聚合酶链反应呈阳性。
