de Fúcio Suzana Beatriz Portugal, Puppin-Rontani Regina Maria, de Carvalho Fabíola Galbiatti, Mattos-Graner Renata de Oliveira, Correr-Sobrinho Lourenço, Garcia-Godoy Franklin
Department of Pediatric Dentistry, Piracicaba Dental School, Unicamp, Av. Limeira 901, 13414-018 Piracicaba, SP, Brazil.
Am J Dent. 2009 Jun;22(3):131-6.
To qualitatively and quantitatively assess the architectural arrangement of microorganisms in biofilm developed on the surface of different restorative materials: ceramic (C), resin composite (RC), conventional (CGIC) and resin-modified glass-ionomer cements (RMGIC).
Streptococcus mutans was used to develop a biofilm that adhered to the surfaces of the selected material disks in 30 days. The specimens were stained and analyzed by confocal laser scanning microscopy and COMSTAT. Among biofilm properties, mean thickness, total bio-volume, roughness coefficient and surface-to-volume ratio were investigated, as well as characteristics of the distribution and architecture of viable/nonviable cells in the biofilm.
Only the mean biofilm thickness was statistically significantly different among the restorative materials tested. C and RC accumulated the thickest biofilms. Qualitative analysis showed cellular aggregates and fluid-filled channels penetrating to a considerable depth of the biofilm. In addition, images demonstrated a progression of more viable cells in superficial regions of the biofilm to proportionally more nonviable cells in the deeper regions of the biofilms near the disk.
定性和定量评估在不同修复材料表面形成的生物膜中微生物的结构排列，这些修复材料包括陶瓷（C）、树脂复合材料（RC）、传统玻璃离子水门汀（CGIC）和树脂改性玻璃离子水门汀（RMGIC）。
使用变形链球菌在30天内形成附着在所选材料圆盘表面的生物膜。对标本进行染色，并通过共聚焦激光扫描显微镜和COMSTAT进行分析。研究了生物膜的特性，包括平均厚度、总体积、粗糙度系数和表面积与体积比，以及生物膜中活细胞/死细胞的分布和结构特征。
在所测试的修复材料中，只有生物膜的平均厚度存在统计学显著差异。C和RC积累的生物膜最厚。定性分析显示细胞聚集体和充满液体的通道深入到生物膜相当深的部位。此外，图像显示生物膜表面区域活细胞逐渐增多，而靠近圆盘的生物膜较深区域死细胞比例相应增加。
