Department of Molecular Biotechnology, Da-Yeh University, Changhua, Taiwan.
J Agric Food Chem. 2009 Aug 26;57(16):7288-92. doi: 10.1021/jf901405c.
The biocontrol fungal species of Trichoderma, which colonizes plant roots, are well-known for their potential to control plant pathogens. Six anthraquinones, of which four have been identified for the first time from Trichoderma and two have already been reported in other strains, were purified from Trichoderma harzianum strain Th-R16 to evaluate their biological activities. The structures of the compounds were determined by one- and two-dimensional NMR. The compounds were shown to exhibit stronger antifungal activity than antibacterial activity. Low yield compounds, like 1,5-dihydroxy-3-hydroxymethyl-9,10-anthraquinone, were found to be more active against fungal pathogens than pachybasin and crysophanol, which were found to be the major extracellular metabolites. Test anthraquinones with higher oxidation numbers had better antifungal activity, and their activities were concentration-dependent.
从哈茨木霉菌株 Th-R16 中分离到六种蒽醌类化合物，其中四种为该菌首次发现，两种已在其他菌株中报道，对其进行了分离和结构鉴定，以评估其生物活性。通过一维和二维 NMR 确定了化合物的结构。结果表明，这些化合物具有较强的抗真菌活性，而抗菌活性较弱。低产化合物，如 1,5-二羟基-3-羟甲基-9,10-蒽醌，对真菌病原体的活性高于主要胞外代谢产物 pachybasin 和 crysophanol。具有较高氧化数的测试蒽醌具有更好的抗真菌活性，其活性与浓度有关。
