Liu Shu-Ying, Lo Chaur-Tsuen, Chen Chinpiao, Liu Ming-Yih, Chen Jun-Hui, Peng Kou-Cheng
Department of Molecular Biotechnology, Da-Yeh University, Changhua 51591, Taiwan.
J Biochem Biophys Methods. 2007 Apr 10;70(3):391-5. doi: 10.1016/j.jbbm.2006.09.003. Epub 2006 Sep 16.
Anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from Trichoderma harzianum ETS 323. The fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. Structure of chrysophanol was solved by X-ray diffraction and pachybasin by NMR spectra. About 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/-0.2% and 5.6+/-0.5%, respectively.
从哈茨木霉ETS 323中，通过硅胶柱色谱法，使用两种不同的溶剂系统，纯化得到了蒽醌衍生物大黄酚和厚基孢菌素。该真菌在甘蔗渣固体培养基中于室温下静置培养。大黄酚的结构通过X射线衍射解析，厚基孢菌素的结构通过核磁共振光谱解析。每千克固体培养基可回收约233±13毫克的纯大黄酚和773±40毫克的纯厚基孢菌素，产率分别为1.7±0.2%和5.6±0.5%。
