Willems L, Kerkhofs P, Attenelle L, Burny A, Portetelle D, Kettmann R
Molecular Biology Unit, Faculty of Agronomy, Gembloux, Belgium.
J Gen Virol. 1997 Mar;78 ( Pt 3):637-40. doi: 10.1099/0022-1317-78-3-637.
In order to gain insight into the role of the major homology region (MHR) in the infectious potential of bovine leukaemia virus (BLV), mutations were introduced into the capsid gene of an infectious molecular clone. A provirus that was designed to contain only a slightly modified version of the MHR (substitution of phenylalanine 147 with a tyrosine) was still infectious in vivo. Furthermore, the provirus loads were not significantly different from those obtained with a wild-type virus. A second mutant was designed to analyse a mild modification of the MHR at the level of arginine 150. The substitution of this residue with a lysine completely destroyed the infectious potential of the recombinant virus. Finally, a third mutant that was deleted in the MHR region was unable to infect the host. Thus it appears that the integrity of the MHR domain is essential for BLV infectivity in vivo.
为了深入了解主要同源区域（MHR）在牛白血病病毒（BLV）感染潜力中的作用，将突变引入到感染性分子克隆的衣壳基因中。一种设计为仅包含MHR轻微修饰版本（用酪氨酸取代苯丙氨酸147）的前病毒在体内仍具有感染性。此外，前病毒载量与野生型病毒获得的载量没有显著差异。设计了第二个突变体以分析MHR在精氨酸150水平的轻微修饰。用赖氨酸取代该残基完全破坏了重组病毒的感染潜力。最后，在MHR区域缺失的第三个突变体无法感染宿主。因此，MHR结构域的完整性对于BLV在体内的感染性似乎至关重要。
