Department of Life & Environmental Sciences, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea ; Cell Activation Research Institute, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:879696. doi: 10.1155/2013/879696. Epub 2013 Apr 24.
Rhus verniciflua Stokes (RV) has traditionally been used as a food supplement and a traditional herbal medicine for centuries in Korea. Recent studies suggest that RV has potent antioxidative, antitumor, and anti-inflammatory properties. In this study, the anti-inflammatory effects of RV from mice sensitized with 2,4-dinitrofluorobenzene (DNFB) and activated macrophages were investigated. The results showed that RV reduced ear swelling and hyperplasia of ear tissue as well as an increase in vascular permeability, which are characteristics of allergic contact dermatitis (ACD) with evident histomorphological changes in epidermis and dermis. Decreased numbers of infiltrated mast cells were seen in RV extract treated group, using toluidine blue staining. RV extract significantly regulates the expression of inducible nitric oxide synthase (iNOS) at the translational level in activated macrophages. Furthermore, RV extract and its active compound, fisetin, attenuated the level of tumor necrosis factor- α (TNF- α ) and interleukin 6 (IL-6) mRNA in LPS-stimulated macrophages. Anti-ACD effect of RV extract may be due to the suppression of iNOS and proinflammatory cytokines which might be mediated via the NF κ B signaling pathways. Collectively, RV extract has potential for alleviating ACD-like symptoms induced by DNFB in the mouse.
漆树（Rhus verniciflua Stokes，RV）在韩国已被作为食品补充剂和传统草药使用了几个世纪。最近的研究表明，漆树具有很强的抗氧化、抗肿瘤和抗炎特性。在这项研究中，研究了用 2,4-二硝基氟苯（DNFB）敏化和激活巨噬细胞的小鼠中漆树的抗炎作用。结果表明，漆树可减少耳朵肿胀和耳朵组织增生以及血管通透性增加，这是过敏性接触性皮炎（ACD）的特征，表皮和真皮有明显的组织形态学变化。甲苯胺蓝染色显示，漆树提取物处理组的浸润肥大细胞数量减少。漆树提取物在翻译水平上显著调节激活巨噬细胞中诱导型一氧化氮合酶（iNOS）的表达。此外，漆树提取物及其活性化合物非瑟酮可减弱 LPS 刺激的巨噬细胞中肿瘤坏死因子-α（TNF-α）和白细胞介素 6（IL-6）mRNA 的水平。漆树提取物的抗 ACD 作用可能是由于抑制 iNOS 和促炎细胞因子，这可能是通过 NF κ B 信号通路介导的。总之，漆树提取物具有减轻小鼠中由 DNFB 引起的 ACD 样症状的潜力。
